Abstract | BACKGROUND: METHODS for predicting the clinical outcome of specific oral immunotherapy (OIT) would improve the safety of the therapy. METHODS: We investigated 40 children aged 6-17 years with IgE-mediated cow's milk allergy (CMA) who either successfully completed OIT (n = 32) or discontinued the therapy due to adverse reactions (n = 8). From sera drawn before and after OIT, we analyzed specific IgA, IgG, IgG1 and IgG4 to cow's milk (CM), casein, β- lactoglobulin and ovalbumin (with enzyme-linked immunosorbent assay) and IgE to CM and hen's egg white [with enzymatic fluoroimmunoassay (Phadia ThermoFisher Scientific CAP system)]. As a reference, we also analyzed serum samples from 8- to 9-year-old children who either had no history of CMA (n = 76) or who had spontaneously recovered from IgE-mediated CMA (n = 56). RESULTS: Levels of specific IgA, IgG, IgG1 and IgG4 to CM and casein, and CM-specific IgE prior to OIT were higher in children who discontinued the therapy than in those who achieved desensitization (p < 0.05). Adverse reactions in the entire population were associated with low IgG and IgG4, but high IgG1 levels to ovalbumin (p < 0.05). Specific IgA, IgG, IgG1 and IgG4 to CM proteins significantly increased and IgE to CM decreased during OIT in children who achieved desensitization (p < 0.01). In those who discontinued OIT, only IgG, IgG1 and IgG4 to CM increased significantly (p < 0.03) and CM IgE remained unchanged. CONCLUSIONS:
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Authors | Emma M Savilahti, Mikael Kuitunen, Erkki Savilahti, Mika J Mäkelä |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 164
Issue 1
Pg. 32-9
( 2014)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 24853260
(Publication Type: Journal Article)
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Copyright | © 2014 S. Karger AG, Basel. |
Chemical References |
- Caseins
- Immunoglobulin A
- Immunoglobulin G
- Lactoglobulins
- Ovalbumin
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Topics |
- Administration, Oral
- Adolescent
- Animals
- Caseins
(immunology)
- Child
- Desensitization, Immunologic
(methods)
- Female
- Humans
- Immunoassay
- Immunoglobulin A
(blood, immunology)
- Immunoglobulin G
(blood, immunology)
- Kinetics
- Lactoglobulins
(immunology)
- Male
- Milk
(immunology)
- Milk Hypersensitivity
(blood, immunology, prevention & control)
- Ovalbumin
(immunology)
- Treatment Outcome
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