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Regulation of GTP cyclohydrolase I and dihydropteridine reductase in rat pheochromocytoma PC 12 cells.

Abstract
The addition of 8-bromo cyclic AMP, forskolin, theophylline, and 3-isobutyl-1-methylxanthine to the medium of PC 12 cells resulted in an increase in GTP cyclohydrolase I activity, but had no effect on dihydropteridine reductase activity, except theophylline which caused a decrease in dihydropteridine reductase activity at 96 h. GTP cyclohydrolase I activity peaked at 24 h and returned to normal 96 h after drug treatment. Cycloheximide decreased GTP cyclohydrolase I activity at 48 and 96 h, but had little effect on dihydropteridine reductase activity. The addition of reserpine selectively increased only GTP cyclohydrolase I activity. The addition of tetrahydrobiopterin and sepiapterin, however, coordinately inhibited both GTP cyclohydrolase I and dihydropteridine reductase activities. It appears that GTP cyclohydrolase I activity in PC 12 cells is regulated by cyclic AMP stimulation and by end-product inhibition, whereas dihydropteridine reductase activity is only subject to pterin inhibition.
AuthorsR S Shen, Y X Zhang, J R Perez-Polo
JournalJournal of enzyme inhibition (J Enzyme Inhib) Vol. 3 Issue 2 Pg. 119-26 ( 1989) ISSN: 8755-5093 [Print] Switzerland
PMID2484967 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Isoenzymes
  • Pteridines
  • Pterins
  • Colforsin
  • Biopterin
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Reserpine
  • Cycloheximide
  • Theophylline
  • sepiapterin
  • Dihydropteridine Reductase
  • GTP Cyclohydrolase
  • sapropterin
  • 1-Methyl-3-isobutylxanthine
Topics
  • 1-Methyl-3-isobutylxanthine (pharmacology)
  • 8-Bromo Cyclic Adenosine Monophosphate (pharmacology)
  • Adrenal Gland Neoplasms
  • Animals
  • Biopterin (analogs & derivatives, pharmacology)
  • Cell Line
  • Colforsin (pharmacology)
  • Cycloheximide (pharmacology)
  • Dihydropteridine Reductase (metabolism)
  • GTP Cyclohydrolase (metabolism)
  • Isoenzymes (metabolism)
  • Kinetics
  • Pheochromocytoma
  • Pteridines (pharmacology)
  • Pterins
  • Rats
  • Reserpine (pharmacology)
  • Theophylline (pharmacology)

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