Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma.

Abstract

The present work aimed to investigate the expression and role of aminoacylase 1 (ACY1) in hepatocellular carcinoma (HCC) based on a proteomic study. The study results revealed that the expression of ACY1 was much lower in HCC tissues. ACY1 expression significantly correlated with the serum alpha fetoprotein level and tumor invasiveness. The knockdown of ACY1 in SMMC7721 cells promoted cell viability and invasiveness. In contrast, the restoration of ACY1 in BEL7402 cells inhibited cell viability and invasiveness. Further studies indicated that the knockdown of ACY1 increased the protein expression of transforming growth factor beta 1 and extracellular signal-regulated kinase 1 expression. The study's results indicated that ACY1 acts as a tumor suppressor in HCC.

• Authors: Xuyong Wei, Jie Li, Haiyang Xie, Qi Ling, Jianguo Wang, Di Lu, Lin Zhou, Xiao Xu, Shusen Zheng
• Journal: Cancer letters (Cancer Lett) Vol. 351 Issue 1 Pg. 117-25 (Aug 28 2014) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 24846301 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Proteome
• Tumor Suppressor Proteins
• Amidohydrolases
• aminoacylase I

Topics

• Amidohydrolases (physiology)
• Apoptosis
• Carcinoma, Hepatocellular (enzymology)
• Female
• Gene Knockdown Techniques
• Hep G2 Cells
• Humans
• Liver Neoplasms (enzymology)
• Male
• Middle Aged
• Proteome (metabolism)
• Proteomics
• S Phase Cell Cycle Checkpoints
• Tandem Mass Spectrometry
• Tumor Suppressor Proteins (physiology)
• Two-Dimensional Difference Gel Electrophoresis