Inactivation of dengue virus in plasma with amotosalen and ultraviolet A illumination.

Abstract

BACKGROUND:

Dengue virus (DENV) is the most prevalent arbovirus in tropical and subtropical regions. Transfusion-transmitted DENV infections have already been reported and the risk for blood products to be contaminated by DENV needs to be considered in dengue-endemic areas, especially during outbreaks. Blood product inactivation processes, including amotosalen and ultraviolet A (UVA) illumination, have been developed to reduce transfusion-transmitted infections. In this study we demonstrate the efficiency of using amotosalen and UVA illumination for DENV inactivation in human plasma.

STUDY DESIGN AND METHODS:

Plasma units from volunteer blood donors were spiked with DENV. Viral titers and viral RNA loads were measured in plasma before and after amotosalen and UVA photochemical treatment.

RESULTS:

The mean DENV titer in plasma before inactivation was 5.61 log 50% tissue culture infectious dose (TCID50)/mL and the mean viral RNA load was 10.21 log copies/mL. In inactivated plasma, the mean DENV RNA load was 9.37 log copies/mL, but cell cultures inoculated with inactivated plasma did not result in infected cells and did not produce any replicative virus nor detectable viral RNA.

CONCLUSION:

We report here that amotosalen combined with UVA light inactivated DENV in fresh-frozen plasma (5.61 log inactivation of viral titer). This inactivation process is an efficient method to prevent plasma transfusion-transmitted DENV infections.

• Authors: Didier Musso, Vaea Richard, Julien Broult, Van-Mai Cao-Lormeau
• Journal: Transfusion (Transfusion) Vol. 54 Issue 11 Pg. 2924-30 (Nov 2014) ISSN: 1537-2995 [Electronic] United States
• PMID: 24845685 (Publication Type: Clinical Trial, Journal Article)
• Copyright: © 2014 AABB.

Chemical References

• Furocoumarins
• Photosensitizing Agents
• RNA, Viral
• amotosalen

Topics

• Blood Component Transfusion
• Blood Donors
• Blood Safety
• Dengue (prevention & control, transmission)
• Dengue Virus
• Female
• Furocoumarins (pharmacology)
• Humans
• Male
• Photosensitizing Agents (pharmacology)
• Plasma (virology)
• Polynesia
• RNA, Viral (blood)
• Ultraviolet Rays
• Virus Inactivation (drug effects, radiation effects)