Abstract | PURPOSE: MATERIALS AND METHODS: An animal model of renal interstitial fibrosis was established using unilateral ureteral obstruction with enalapril as the positive control. Rats were randomly divided into 6 groups, including sham treated, model, enalapril, and high, medium and low stachydrine. On day 14 postoperatively the rats were sacrificed. Serum was collected to determine serum creatinine and blood urea nitrogen. Tubular injury index was measured by hematoxylin and eosin staining. Renal interstitial collagen deposition was analyzed semiquantitatively by Masson staining. Expression of the apoptotic factors caspase-12 and 9 in renal tissues was determined by immunohistochemistry. RESULTS: CONCLUSIONS:
Stachydrine interfered with the endoplasmic reticulum stress mediated apoptosis pathway by decreasing caspase-12 expression and inhibiting caspase-9 activation. Ultimately renal tubular epithelial cell apoptosis was suppressed and renal interstitial fibrosis development was postponed.
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Authors | Cui Zhang, Ying Lu, Ya-Jie Zhou, Qian-Qian Tong, Chao Qu, Tian-Ji Kang |
Journal | The Journal of urology
(J Urol)
Vol. 192
Issue 5
Pg. 1549-54
(Nov 2014)
ISSN: 1527-3792 [Electronic] United States |
PMID | 24840537
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 American Urological Association Education and Research, Inc. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Biomarkers
- Proline
- Caspase 12
- Caspase 9
- stachydrine
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Topics |
- Animals
- Apoptosis
(drug effects)
- Biomarkers
(metabolism)
- Blood Urea Nitrogen
- Caspase 12
(biosynthesis)
- Caspase 9
(biosynthesis)
- Disease Models, Animal
- Immunohistochemistry
- Kidney
(pathology)
- Male
- Proline
(analogs & derivatives, pharmacology)
- Rabbits
- Rats
- Rats, Wistar
- Ureteral Obstruction
(enzymology, pathology, therapy)
- Urologic Surgical Procedures, Male
(methods)
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