Glutathione S-transferase gene polymorphisms (GSTM1, GSTT1, and GSTP1) in Egyptian pediatric patients with sickle cell disease.

Abstract	Sickle cell disease (SCD) complications are associated with oxidative stress. Glutathione S-transferases (GSTs) are a group of enzymes that protect against oxidative stress. The aims of this study was to evaluate the prevalence of GSTM1, GSTT1, and GSTP1 gene polymorphisms among homozygous sickle cell anemia patients and to investigate the possible association between the presence of these polymorphisms and SCD severity and complications. Genotyping the polymorphisms in GSTT1 and GSTM1 genes was performed using the multiplex polymerase chain reaction (PCR) method. The GSTP1 ILe105Val polymorphism was determined using PCR-restriction fragment length polymorphism. GSTM1 null genotype was significantly associated with increased risk of severe vaso-occlusive crises (VOC) (odds ratio = 1.52, 95% confidence interval = 0.42-5.56, P = 0.005). We found no significant association between GST genotypes and frequency of sickle cell-related pain, transfusion frequency, disease severity, or hydroxyurea treatment. GSTM1 gene polymorphism may be associated with risk of severe VOC among Egyptian SCD patients.
Authors	Hala Fathy Shiba, Mona Kamal El-Ghamrawy, Iman Abd El-Mohsen Shaheen, Rasha Abd El-Ghani Ali, Somaia Mohammed Mousa
Journal	Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society (Pediatr Dev Pathol) 2014 Jul-Aug Vol. 17 Issue 4 Pg. 265-70 ISSN: 1093-5266 [Print] United States
PMID	24840051 (Publication Type: Journal Article)
Chemical References	Antisickling Agents glutathione S-transferase T1 GSTP1 protein, human Glutathione S-Transferase pi Glutathione Transferase glutathione S-transferase M1 Hydroxyurea
Topics	Adolescent Age Factors Anemia, Sickle Cell (complications, diagnosis, drug therapy, enzymology, genetics) Antisickling Agents (therapeutic use) Case-Control Studies Chi-Square Distribution Child Child, Preschool Cross-Sectional Studies Disease Progression Egypt Female Gene Frequency Genetic Predisposition to Disease Glutathione S-Transferase pi (genetics) Glutathione Transferase (genetics) Homozygote Humans Hydroxyurea (therapeutic use) Male Odds Ratio Pain Measurement Phenotype Polymerase Chain Reaction Polymorphism, Genetic Prognosis Risk Assessment Risk Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!