Abstract | BACKGROUND AIMS: METHODS: In the present study, we performed a detailed analysis of the distribution of peripheral blood subsets of T, B and natural killer (NK) lymphocytes in 23 patients with Fanconi anemia before and after BMT on days +30, +60, +100, +180, +270 and +360. In parallel, we evaluated the effect of related versus unrelated donor marrow as well as the presence of graft-versus-host disease (GVHD). RESULTS: After transplantation, we found different kinetics of recovery for the distinct major subsets of lymphocytes. NK cells were the first to recover, followed by cytotoxic CD8(+) T cells and B cells, and finally CD4(+) helper T cells. Early lymphocyte recovery was at the expense of memory cells, potentially derived from the graft, whereas recent thymic emigrant (CD31(+) CD45RA(+)) and naive CD4(+) or CD8(+) T cells rose only at 6 months after HSCT, in the presence of immunosuppressive GVHD prophylactic agents. Only slight differences were observed in the early recovery of cytotoxic CD8(+) T cells among those cases receiving a graft from a related donor versus an unrelated donor. Patients with GVHD displayed a markedly delayed recovery of NK cells and B cells as well as of regulatory T cells and both early thymic emigrant and total CD4(+) T cells. CONCLUSIONS: Our results support the utility of post-transplant monitoring of a peripheral blood lymphocyte subset for improved follow-up of patients with Fanconi anemia undergoing BMT.
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Authors | Miriam Perlingeiro Beltrame, Mariester Malvezzi, Carmem Bonfim, Dimas Tadeu Covas, Alberto Orfao, Ricardo Pasquini |
Journal | Cytotherapy
(Cytotherapy)
Vol. 16
Issue 7
Pg. 976-89
(Jul 2014)
ISSN: 1477-2566 [Electronic] England |
PMID | 24831839
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. |
Topics |
- Adolescent
- Adult
- Bone Marrow Transplantation
(methods)
- CD8-Positive T-Lymphocytes
(immunology)
- Child
- Child, Preschool
- Fanconi Anemia
(genetics, immunology, pathology, therapy)
- Female
- Graft vs Host Disease
(genetics, immunology)
- Humans
- Killer Cells, Natural
(transplantation)
- Male
- Transplantation, Homologous
(methods)
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