Abstract |
Vinblastine and vincristine, both of which are bisindole alkaloids derived from vindoline and catharanthine, have been used for cancer chemotherapy; their monomeric precursor molecules are vindoline and catharanthine. A simple and selective liquid chromatography mass spectrometry method for simultaneous determination of vindoline and catharanthine in rat plasma was developed. Chromatographic separation was achieved on a C18 (2.1 × 50 mm, 3.5 µm) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. The flow rate was set at 0.4 mL/min. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification. Mean recoveries were in the range of 87.3-92.6% for vindoline in rat plasma and 88.5-96.5% for catharanthine. Matrix effects for vindoline and catharanthine were measured to be between 95.3 and 104.7%. Coefficients of variation of intra-day and inter-day precision were both <15%. The accuracy of the method ranged from 93.8 to 108.1%. The method was successfully applied in a pharmacokinetic study of vindoline and catharanthine in rats. The bioavailability of vindoline and catharanthine were 5.4 and 4.7%, respectively.
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Authors | Chongliang Lin, Jinzhang Cai, Xuezhi Yang, Lufeng Hu, Guanyang Lin |
Journal | Biomedical chromatography : BMC
(Biomed Chromatogr)
Vol. 29
Issue 1
Pg. 97-102
(Jan 2015)
ISSN: 1099-0801 [Electronic] England |
PMID | 24828449
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 John Wiley & Sons, Ltd. |
Chemical References |
- Vinca Alkaloids
- vindoline
- Vinblastine
- catharanthine
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Topics |
- Administration, Intravenous
- Administration, Oral
- Animals
- Biological Availability
- Chromatography, Liquid
(methods)
- Linear Models
- Male
- Mass Spectrometry
(methods)
- Rats
- Rats, Sprague-Dawley
- Reproducibility of Results
- Sensitivity and Specificity
- Vinblastine
(administration & dosage, analogs & derivatives, blood, chemistry, pharmacokinetics)
- Vinca Alkaloids
(administration & dosage, blood, chemistry, pharmacokinetics)
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