HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Liquid chromatography mass spectrometry simultaneous determination of vindoline and catharanthine in rat plasma and its application to a pharmacokinetic study.

Abstract
Vinblastine and vincristine, both of which are bisindole alkaloids derived from vindoline and catharanthine, have been used for cancer chemotherapy; their monomeric precursor molecules are vindoline and catharanthine. A simple and selective liquid chromatography mass spectrometry method for simultaneous determination of vindoline and catharanthine in rat plasma was developed. Chromatographic separation was achieved on a C18 (2.1 × 50 mm, 3.5 µm) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. The flow rate was set at 0.4 mL/min. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification. Mean recoveries were in the range of 87.3-92.6% for vindoline in rat plasma and 88.5-96.5% for catharanthine. Matrix effects for vindoline and catharanthine were measured to be between 95.3 and 104.7%. Coefficients of variation of intra-day and inter-day precision were both <15%. The accuracy of the method ranged from 93.8 to 108.1%. The method was successfully applied in a pharmacokinetic study of vindoline and catharanthine in rats. The bioavailability of vindoline and catharanthine were 5.4 and 4.7%, respectively.
AuthorsChongliang Lin, Jinzhang Cai, Xuezhi Yang, Lufeng Hu, Guanyang Lin
JournalBiomedical chromatography : BMC (Biomed Chromatogr) Vol. 29 Issue 1 Pg. 97-102 (Jan 2015) ISSN: 1099-0801 [Electronic] England
PMID24828449 (Publication Type: Journal Article)
CopyrightCopyright © 2014 John Wiley & Sons, Ltd.
Chemical References
  • Vinca Alkaloids
  • vindoline
  • Vinblastine
  • catharanthine
Topics
  • Administration, Intravenous
  • Administration, Oral
  • Animals
  • Biological Availability
  • Chromatography, Liquid (methods)
  • Linear Models
  • Male
  • Mass Spectrometry (methods)
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Vinblastine (administration & dosage, analogs & derivatives, blood, chemistry, pharmacokinetics)
  • Vinca Alkaloids (administration & dosage, blood, chemistry, pharmacokinetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: