Abstract | OBJECTIVE: METHOD: Human salivary in adenoid cystic carcinoma cell line AC-M was cultured, divided into the experimental group (5-FU group, garlic oil group, garlic oil + 5-FU group) and the control group, to observe the growth activity of tumor cells by MTT methods; to analyse the changes of cell cycle and apoptosis rate by flow cytometry. RESULT: MTT experiments showed that 5-FU, garlic oil, garlic oil and 5-FU on ACC-M cells have inhibition in different concentration, with the increase of concentration and action time of the rise; Cell cycle analysis showed significant changes in flow cytometry. With the increase of concentration and the acting time, the G0/G1, phase of the cell ratio increased, S had no significant change, but G2/M phase cells decreased. Apoptosis rate display showed garlic oil combined with 5-FU induced apoptosis of ACC-M cells was significantly stronger than single group. CONCLUSION:
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Authors | Fayin Wu, Hefeng Zhou, Zhiying Fan, Yawen Zhu, Yongye Li, Yukun Yao, Dan Ran |
Journal | Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery
(Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi)
Vol. 28
Issue 3
Pg. 175-7, 181
(Feb 2014)
ISSN: 2096-7993 [Print] China |
PMID | 24826454
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Allyl Compounds
- Sulfides
- allyl sulfide
- Fluorouracil
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Topics |
- Allyl Compounds
(pharmacology)
- Apoptosis
(drug effects)
- Carcinoma, Adenoid Cystic
(pathology)
- Cell Line, Tumor
- Fluorouracil
(pharmacology)
- Humans
- Sulfides
(pharmacology)
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