Protective effect of salidroside on cardiac apoptosis in mice with chronic intermittent hypoxia.

Abstract

BACKGROUND:

The goal of this study is to determine if salidroside has protective effects on hypoxia-induced cardiac widely dispersed apoptosis in mice with severe sleep apnea model.

METHODS:

Sixty-four C57BL/6J mice 5-6 months of age were divided into four groups, i.e. Control group (21% O2, 24h per day, 8 weeks, n=16); Hypoxia group (Hypoxia: 7% O2 60s, 20% O2 alternating 60s, 8h per day, 8 weeks, n=16); and Hypoxia+S10 and Hypoxia+S 30 groups (Hypoxia for 1st 4 weeks, hypoxia pretreated 10mg/kg and 30 mg/kg salidroside by oral gavage per day for 2nd 4 weeks, n=16 and 16). The excised hearts from four groups were measured by the heart weight index, H&E staining, TUNEL-positive assays and Western blotting.

RESULTS:

TUNEL-positive apoptotic cells in mice heart were less in Hypoxia+S10 and Hypoxia+S30 than those in the Hypoxia group. Compared with Hypoxia, the protein levels of Fas ligand, Fas death receptors, Fas-Associated Death Domain (FADD), activated caspase 8, and activated caspase 3 (Fas pathways) were decreased in Hypoxia+S10 and Hypoxia+S30. In the mitochondria pathway, the protein levels of BcLx, Bcl2, and Bid (anti-apoptotic Bcl2 family) in Hypoxia+S10 and Hypoxia+S30 were more than those in Hypoxia. The protein levels of Bax, t-Bid, activated caspase 9, and activated caspase 3 were less in Hypoxia+S10 and Hypoxia+S30 than those in hypoxia.

CONCLUSIONS:

Our findings suggest that salidroside has protective effects on chronic intermittent hypoxia-induced Fas-dependent and mitochondria-dependent apoptotic pathways in mice hearts.

• Authors: Mei-Chih Lai, Jaung-Geng Lin, Pei-Ying Pai, Mei-Hsin Lai, Yueh-Min Lin, Yu-Lan Yeh, Shiu-Min Cheng, Yi-fan Liu, Chih-Yang Huang, Shin-Da Lee
• Journal: International journal of cardiology (Int J Cardiol) Vol. 174 Issue 3 Pg. 565-73 (Jul 01 2014) ISSN: 1874-1754 [Electronic] Netherlands
• PMID: 24825027 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Cardiotonic Agents
• Glucosides
• Phenols
• rhodioloside

Topics

• Animals
• Apoptosis (drug effects, physiology)
• Cardiotonic Agents (pharmacology, therapeutic use)
• Chronic Disease
• Glucosides (pharmacology, therapeutic use)
• Heart (drug effects)
• Hypoxia (drug therapy, pathology)
• Male
• Mice
• Mice, Inbred C57BL
• Mitochondria, Heart (drug effects, pathology)
• Phenols (pharmacology, therapeutic use)
• Rhodiola