Annexin A3 participates in various biological processes, including
tumorigenesis, drug resistance, and
metastasis. The aim of this study was to investigate the expression of
Annexin A3 in
gastric cancer and its relationship with cell differentiation, migration, and invasion of
gastric cancer cells.
Annexin A3 expression in
gastric cancer tissues was detected by quantitative real-time PCR and Western blotting. The proliferation of
gastric cancer cells was measured by the MTT assay. Cell migration and invasion were determined via wound healing and transwell assays, respectively. Knock down of endogenous
Annexin A3 in
gastric cancer BGC823 cells was performed using
siRNA technology. The expression of
Annexin A3 was significantly upregulated in
gastric cancer tissues, and negatively correlated with the differentiation degree. Silencing of endogenous
Annexin A3 suppressed the proliferation, migration, and invasion of BGC823 cells. Additionally, the expression of p21, p27,
TIMP-1, and
TIMP-2 was upregulated, and the expression of
PCNA,
cyclin D1, MMP-1, and MMP-2 decreased in cells treated with
Annexin A3-siRNA.
Annexin A3 was upregulated in
gastric cancer cells. Deletion of endogenous
Annexin A3 significantly inhibited
gastric cancer cell proliferation, migration, and invasion.