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Renal epithelial fluid secretion and cyst growth: the role of cyclic AMP.

Abstract
Transepithelial fluid secretion has been postulated to account for the accumulation of fluid within hereditary and acquired renal cysts, but no such mechanism has been demonstrated in human kidney epithelium. It is shown here that transepithelial fluid secretion was stimulated by prostaglandin E1 (PGE1), forskolin, 8-Br-cyclic AMP, and 1-methyl-3-isobutylxanthine in polarized monolayers of established renal cell lines (MDCK and rat glomerular epithelial cells) and in monolayer cultures derived from the cyst walls of human autosomal dominant polycystic kidney disease and from epithelial cells of normal human renal cortex. Treatment with cyclic AMP agonists caused the same cells, when dispersed within a gel matrix of type I collagen (Vitrogen), to proliferate and form spherical fluid-filled monolayered cysts. Our findings suggest that increased intracellular cyclic AMP levels may have a critical role in the formation and expansion of hereditary and acquired renal cysts.
AuthorsR Mangoo-Karim, M E Uchic, M Grant, W A Shumate, J P Calvet, C H Park, J J Grantham
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology (FASEB J) Vol. 3 Issue 14 Pg. 2629-32 (Dec 1989) ISSN: 0892-6638 [Print] United States
PMID2480260 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Colforsin
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Cyclic AMP
  • Alprostadil
  • 1-Methyl-3-isobutylxanthine
Topics
  • 1-Methyl-3-isobutylxanthine (pharmacology)
  • 8-Bromo Cyclic Adenosine Monophosphate (pharmacology)
  • Alprostadil (pharmacology)
  • Animals
  • Body Fluids (metabolism)
  • Cell Line
  • Colforsin (pharmacology)
  • Cyclic AMP (physiology)
  • Dogs
  • Epithelium (drug effects, metabolism, pathology)
  • Humans
  • Kidney (drug effects, metabolism, pathology)
  • Kidney Diseases, Cystic (pathology, physiopathology)
  • Rats

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