Poligapolide, a PI3K/Akt inhibitor in immunodeficiency virus type 1 TAT-transduced CHME5 cells, isolated from the rhizome of Polygala tenuifolia.

Abstract

The rhizome of Polygala tenuifolia WILLD (PT, family Polygalaceae) has been used in traditional Chinese medicine for inflammation, dementia, amnesia, neurasthenia and cancer. The phosphoinositide 3-kinase (PI3K)/Akt inhibitor(s) was isolated from PT by using the cytoprotective phenotype of human immunodeficiency virus type 1 (HIV-1) Tat-transduced CHME5 cells against lipopolysaccharide/cycloheximide. We isolated 9 constituents (1)-(9) from ethyl acetate fraction of PT, which potently showed anti-cytoprotective effect against HIV-1 TAT-transduced cells. Of them, (9R)-(-)-9-peptandecanolide (2), a new compound named poligapolide, most potently abolished the cytoprotective effect of HIV-1 Tat-transduced CHME5 cells. The compound (2) inhibited the phosphorylation of Akt and its downstream molecule, glycogen synthase kinase-3 beta (GSK3β) in PI3K/Akt cell survival signaling pathway, but did not suppress the phosphorylation of PI3K and pyruvate dehydrogenase lipoamide kinase isozyme 1. Based on these finding, poligapolide may abolish the cytoprotective phenotype of HIV-1 Tat-transduced CHME5 cells by inhibiting Akt phosphorylation in PI3K/Akt pathway.

• Authors: Sul-Young Yoo, Thi Kim Van Le, Jin Ju Jeong, Dong-Hyun Kim
• Journal: Chemical & pharmaceutical bulletin (Chem Pharm Bull (Tokyo)) Vol. 62 Issue 5 Pg. 467-71 ( 2014) ISSN: 1347-5223 [Electronic] Japan
• PMID: 24789928 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lactones
• Lipopolysaccharides
• Phosphoinositide-3 Kinase Inhibitors
• Protein Kinase Inhibitors
• poligapolide
• tat Gene Products, Human Immunodeficiency Virus
• Cycloheximide
• Proto-Oncogene Proteins c-akt

Topics

• Cell Line
• Cell Survival (drug effects)
• Cycloheximide (antagonists & inhibitors, pharmacology)
• Dose-Response Relationship, Drug
• Humans
• Lactones (chemistry, isolation & purification, pharmacology)
• Lipopolysaccharides (antagonists & inhibitors, pharmacology)
• Molecular Structure
• Phosphatidylinositol 3-Kinases (metabolism)
• Phosphoinositide-3 Kinase Inhibitors
• Polygala (chemistry)
• Protein Kinase Inhibitors (chemistry, isolation & purification, pharmacology)
• Proto-Oncogene Proteins c-akt (antagonists & inhibitors, metabolism)
• Rhizome (chemistry)
• Signal Transduction (drug effects)
• Structure-Activity Relationship
• tat Gene Products, Human Immunodeficiency Virus (genetics, metabolism)