Stewartia koreana (S. koreana) has been used in the treatment of inflammatory diseases, such as acute
gastroenteritis and
aches, in Korean
folk medicine and has been reported to have a number of
biological activities, such as anti-inflammatory activity and the promotion of angiogenesis. In this study, we aimed to determine the effects of S. koreana extract (SKE) and its components on dermal fibroblast growth and migration, and to investigate the wound healing activity of the extract in mice. In vitro experiments revealed that the numbers of SKE-treated cells increased by approximately 2.5-‑ and 3.7-fold with 50 and 100 µg/ml of SKE, respectively. 5-bromo-2'-deoxy-uridine (
BrdU) incorporation was also increased in the SKE-treated cells by 2.3-fold. SKE promoted the migration of human skin fibroblasts and, among the isolated compounds,
hyperin increased the proliferation and migration of the fibroblasts to almost the same degree as SKE. Western blot analysis demonstrated that SKE stimulated the
MEK/ERK1/2 and PI3K/Akt signaling pathways. In in vivo experiments, the SKE-treated
wound lesions of mice decreased by approximately 7% in diameter after 2 days of treatment with SKE compared with the
wound lesions on the 1st day of the experiment. On the 9th day of treatment, the diameter of the lesions was further reduced by approximately 83% in the SKE-treated
wound areas compared with the
wound areas on the 1st day of treatment. Our results demonstrate that
methanol extracts of S. koreana leaves promote the proliferation and migration of skin fibroblasts and possess effective wound healing activity through the activation of the
MEK/ERK1/2 and PI3K/Akt signaling pathways.
Hyperin was identified as an active compound responsible for the stimulation of fibroblast growth and migration.