Shikonin blocks migration and invasion of human breast cancer cells through inhibition of matrix metalloproteinase-9 activation.

Abstract

Shikonin, a natural naphthoquinone isolated from a traditional Chinese medicinal herb, has been reported to promote tumor cell death. However, there are few reports concerning its effect on metastasis-related cell invasion and migration behavior. In the present study, we investigated the effect of shikonin on human breast cancer invasion and migration. We found that shikonin inhibited phorbol 12-myristate 13-acetate (PMA)-induced cell migration and invasion in MCF-7 breast cancer cells, which was correlated with modulation of matrix metalloproteinase-9 (MMP-9) through suppression of both expression and proteolytic and promoter activity. We also found that shikonin inhibited both MMP-9 expression and promoter activity in MDA-MB‑231 cells with high metastatic potential. These results indicated that shikonin induces the suppression of migration and invasion through modulation of MMP-9 in human breast cancer cells. Therefore, shikonin may be a potential anticancer drug for human breast cancer therapy.

• Authors: Soon Young Jang, Jae Koan Lee, Eun Hyang Jang, Seo Young Jeong, Jong-Ho Kim
• Journal: Oncology reports (Oncol Rep) Vol. 31 Issue 6 Pg. 2827-33 (Jun 2014) ISSN: 1791-2431 [Electronic] Greece
• PMID: 24789371 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Matrix Metalloproteinase Inhibitors
• Naphthoquinones
• shikonin
• 4-O-methyl-12-O-tetradecanoylphorbol 13-acetate
• Matrix Metalloproteinase 9
• Tetradecanoylphorbol Acetate

Topics

• Breast Neoplasms (drug therapy, genetics, pathology)
• Cell Movement (drug effects)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• MCF-7 Cells
• Matrix Metalloproteinase 9 (biosynthesis)
• Matrix Metalloproteinase Inhibitors (administration & dosage)
• Naphthoquinones (administration & dosage)
• Neoplasm Invasiveness (genetics)
• Signal Transduction (drug effects)
• Tetradecanoylphorbol Acetate (administration & dosage, analogs & derivatives)