Abstract |
Octopine synthase encoded by the T-DNA (transferred DNA) locus ocs synthesizes N2-(D-1-carboxyethyl)-L-amino acids in octopine-type crown gall tumors. So far, derivatives of only basic amino acids have been isolated. We have detected a glutamine derivative and called it heliopine. Tumors induced by several Ti plasmids with transposon Tn5 insertions in the 3' end of ocs still synthesized small quantities of N2-(1-carboxyethyl)-arginine and N2-(1-carboxyethyl)-glutamine. In addition, N2-(1,3-dicarboxypropyl)-asparagine, which is absent in wild-type octopine tumors, was detected in these tumors. These three imino diacids ( octopine, heliopine, and asparaginopine, respectively, or their isomers) were undetectable in tumors induced by Ti plasmids harboring deletions of the ocs gene. Poly(A)+ RNAs which hybridize to the ocs sequence can also be detected in the ocs::Tn5 tumors; these RNAs, however, were heterogeneous in size and shorter in length than the normal ocs mRNA. These results indicate that mutant ocs products synthesize imino diacids in these ocs::Tn5 tumors.
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Authors | C C Chang, R K Jayaswal, C M Chen, S B Gelvin |
Journal | Journal of bacteriology
(J Bacteriol)
Vol. 171
Issue 11
Pg. 5922-7
(Nov 1989)
ISSN: 0021-9193 [Print] United States |
PMID | 2478521
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA Transposable Elements
- Imino Acids
- RNA, Messenger
- Poly A
- RNA
- Amino Acid Oxidoreductases
- Oxidoreductases Acting on CH-NH Group Donors
- D-octopine dehydrogenase
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Topics |
- Amino Acid Oxidoreductases
(genetics)
- DNA Transposable Elements
- Genes, Bacterial
- Imino Acids
(metabolism)
- Nucleic Acid Hybridization
- Oxidoreductases Acting on CH-NH Group Donors
- Plant Tumors
(microbiology)
- Plants
(genetics)
- Plasmids
- Poly A
(genetics)
- RNA
(genetics)
- RNA, Messenger
- Rhizobium
(enzymology, genetics)
- Transcription, Genetic
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