The acute
peripheral neuropathy induced by
oxaliplatin treatment occurs very frequently and is aggravated by exposure to cold.
Goshajinkigan (GJG), a traditional Japanese (
kampo) medicine, was recently shown to be effective against
oxaliplatin-induced acute neuropathy. However, because the effects of GJG and its mechanism in relation to those of its ingredients and its mechanism are not well understood, we examined the effects of GJG on acute neuropathy. Further, we investigated whether GJG affects the functions and gene expressions of transient receptor potential (TRP) channels using a rat model of
oxaliplatin-induced neuropathy. Administration of
oxaliplatin increased withdrawal responses from cold stimulation, and GJG or
calcium gluconate/
magnesium sulfate significantly inhibited the
oxaliplatin-induced
cold hypersensitivity. Application of
menthol, a TRPA1/TRPM8 agonist, or
allyl isothiocyanate (
AITC), a selective TRPA1 agonist, to the hind paw of
oxaliplatin-treated rats enhanced the nocifensive behaviors evoked by each agonist, whereas
oxaliplatin had no significant effect on nocifensive behaviors evoked by
capsaicin, a TRPV1 agonist. GJG treatment reduced
menthol- or
AITC-evoked withdrawal responses potentiated by
oxaliplatin. Furthermore, GJG suppressed the increase of TRPA1 and TRPM8
mRNA expression induced by
oxaliplatin in dorsal root ganglia. These findings suggest that GJG prevented
oxaliplatin-induced acute
peripheral neuropathy by suppressing functional alteration of TRP channels, especially TRPA1 and TRPM8.