Combined probiotic bacteria promotes intestinal epithelial barrier function in interleukin-10-gene-deficient mice.

Abstract

AIM:

To investigate the protective effects of combinations of probiotic (Bifico) on interleukin (IL)-10-gene-deficient (IL-10 KO) mice and Caco-2 cell monolayers.

METHODS:

IL-10 KO mice were used to assess the benefits of Bifico in vivo. IL-10 KO and control mice received approximately 1.5 × 10(8) cfu/d of Bifico for 4 wk. Colons were then removed and analyzed for epithelial barrier function by Ussing Chamber, while an ELISA was used to evaluate proinflammatory cytokines. The colon epithelial cell line, Caco-2, was used to test the benefit of Bifico in vitro. Enteroinvasive Escherichia coli (EIEC) and the probiotic mixture Bifico, or single probiotic strains, were applied to cultured Caco-2 monolayers. Barrier function was determined by measuring transepithelial electrical resistance and tight junction protein expression.

RESULTS:

Treatment of IL-10 KO mice with Bifico partially restored body weight, colon length, and epithelial barrier integrity to wild-type levels. In addition, IL-10 KO mice receiving Bifico treatment had reduced mucosal secretion of tumor necrosis factor-α and interferon-γ, and attenuated colonic disease. Moreover, treatment of Caco-2 monolayers with Bifico or single-strain probiotics in vitro inhibited EIEC invasion and reduced the secretion of proinflammatory cytokines.

CONCLUSION:

Bifico reduced colon inflammation in IL-10 KO mice, and promoted and improved epithelial-barrier function, enhanced resistance to EIEC invasion, and decreased proinflammatory cytokine secretion.

• Authors: Chen-Zhang Shi, Hong-Qi Chen, Yong Liang, Yang Xia, Yong-Zhi Yang, Jun Yang, Jun-Dong Zhang, Shu-Hai Wang, Jing Liu, Huan-Long Qin
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 20 Issue 16 Pg. 4636-47 (Apr 28 2014) ISSN: 2219-2840 [Electronic] United States
• PMID: 24782616 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• IFNG protein, human
• IL10 protein, mouse
• Inflammation Mediators
• Tight Junction Proteins
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Interferon-gamma

Topics

• Animals
• Body Weight
• Caco-2 Cells
• Colitis (immunology, metabolism, microbiology, pathology, therapy)
• Colon (immunology, metabolism, microbiology, ultrastructure)
• Disease Models, Animal
• Electric Impedance
• Female
• Humans
• Inflammation Mediators (metabolism)
• Interferon-gamma (metabolism)
• Interleukin-10 (deficiency, genetics)
• Intestinal Mucosa (immunology, metabolism, microbiology, ultrastructure)
• Mice, 129 Strain
• Mice, Knockout
• Permeability
• Probiotics
• Tight Junction Proteins (metabolism)
• Tight Junctions (metabolism, microbiology)
• Time Factors
• Tumor Necrosis Factor-alpha (metabolism)