Abstract |
The levels of type X collagen in mineralizing normal chicken epiphyses and nonmineralizing rachitic chicken tibial epiphyses were measured and compared. Qualitative immunoperoxidase studies with anti-chick type X collagen monoclonal antibodies on sections from normal and rachitic cartilage demonstrated that the type X collagen levels in rachitic growth plates are reduced. Northern hybridization of mRNA and biosynthetic studies have confirmed that type X collagen synthesis in rickets is also decreased. In hypocalcemic rickets, the level of type X collagen mRNA is reduced by 80% whereas the level of type X collagen mRNA is only reduced by 50% in normocalcemic rickets. These observations provide additional evidence that type X collagen is involved in the process of cartilage mineralization and also suggest that the partial recovery of type X collagen synthesis in normocalcemic rickets may be related to the elevated plasma concentration of calcium. Calcium concentration may therefore play an important role in the control of type X collagen synthesis.
|
Authors | A P Kwan, I R Dickson, A J Freemont, M E Grant |
Journal | The Journal of cell biology
(J Cell Biol)
Vol. 109
Issue 4 Pt 1
Pg. 1849-56
(Oct 1989)
ISSN: 0021-9525 [Print] United States |
PMID | 2477383
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antibodies, Monoclonal
- RNA
- Collagen
|
Topics |
- Animals
- Antibodies, Monoclonal
- Blotting, Northern
- Chickens
- Collagen
(analysis, biosynthesis, genetics)
- Gene Expression
- Growth Plate
(cytology, metabolism, pathology)
- Immunoenzyme Techniques
- Nucleic Acid Hybridization
- RNA
(genetics, isolation & purification)
- Reference Values
- Rickets
(metabolism, pathology)
|