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Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage.

Abstract
The levels of type X collagen in mineralizing normal chicken epiphyses and nonmineralizing rachitic chicken tibial epiphyses were measured and compared. Qualitative immunoperoxidase studies with anti-chick type X collagen monoclonal antibodies on sections from normal and rachitic cartilage demonstrated that the type X collagen levels in rachitic growth plates are reduced. Northern hybridization of mRNA and biosynthetic studies have confirmed that type X collagen synthesis in rickets is also decreased. In hypocalcemic rickets, the level of type X collagen mRNA is reduced by 80% whereas the level of type X collagen mRNA is only reduced by 50% in normocalcemic rickets. These observations provide additional evidence that type X collagen is involved in the process of cartilage mineralization and also suggest that the partial recovery of type X collagen synthesis in normocalcemic rickets may be related to the elevated plasma concentration of calcium. Calcium concentration may therefore play an important role in the control of type X collagen synthesis.
AuthorsA P Kwan, I R Dickson, A J Freemont, M E Grant
JournalThe Journal of cell biology (J Cell Biol) Vol. 109 Issue 4 Pt 1 Pg. 1849-56 (Oct 1989) ISSN: 0021-9525 [Print] United States
PMID2477383 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • RNA
  • Collagen
Topics
  • Animals
  • Antibodies, Monoclonal
  • Blotting, Northern
  • Chickens
  • Collagen (analysis, biosynthesis, genetics)
  • Gene Expression
  • Growth Plate (cytology, metabolism, pathology)
  • Immunoenzyme Techniques
  • Nucleic Acid Hybridization
  • RNA (genetics, isolation & purification)
  • Reference Values
  • Rickets (metabolism, pathology)

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