Abstract | BACKGROUND: FINDINGS: We performed quantitative proteomics and immunohistochemistry in postmortem brain from genetic AD cases. Electron microscopy was used to characterize ultrastructural features of pathologic aggregates. U1-70k and other snRNPs were biochemically enriched in the insoluble fraction of human brain from subjects with presenilin 1 (PS1) mutations. Aggregates of U1 snRNP-immunoreactivity formed cytoplasmic tangle-like structures in cortex of AD subjects with PS1 and amyloid precursor protein (APP) mutations as well as trisomy 21. Ultrastructural analysis with electron microscopy in an APP mutation case demonstrated snRNP immunogold labeling of paired helical filaments (PHF). CONCLUSIONS: These studies identify U1 snRNP pathologic changes in brain of early onset genetic forms of AD. Since dominant genetic mutations and trisomy 21 result in dysfunctional amyloid processing, the findings suggest that aberrant β- amyloid processing may influence U1 snRNP aggregate formation.
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Authors | Chadwick M Hales, Nicholas T Seyfried, Eric B Dammer, Duc Duong, Hong Yi, Marla Gearing, Juan C Troncoso, Elliott J Mufson, Madhav Thambisetty, Allan I Levey, James J Lah |
Journal | Molecular neurodegeneration
(Mol Neurodegener)
Vol. 9
Pg. 15
(Apr 28 2014)
ISSN: 1750-1326 [Electronic] England |
PMID | 24773620
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amyloid beta-Protein Precursor
- PSEN1 protein, human
- Presenilin-1
- Ribonucleoprotein, U1 Small Nuclear
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Alzheimer Disease
(genetics, metabolism, pathology)
- Amyloid beta-Protein Precursor
(genetics)
- Down Syndrome
(complications)
- Female
- Humans
- Immunohistochemistry
- Male
- Microscopy, Electron, Transmission
- Middle Aged
- Mutation
- Presenilin-1
(genetics)
- Ribonucleoprotein, U1 Small Nuclear
(metabolism)
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