Resting plasma membrane potential of rat peritoneal mast cells is set predominantly by the sodium pump.

Abstract

Changes in the plasma membrane potential of two histamine-releasing cells, rat peritoneal mast cells and basophilic leukemia cells 2H3 (RBL 2H3), were recorded with the potential-sensitive dye bis-oxonol. For mast cells, the presence of ouabain or the absence of K+ increased the fluorescence intensity of bis-oxonol; gramicidin had no effect. For RBL 2H3 cells, the presence of ouabain and the absence of K+ also increased bis-oxonol fluorescence but gramicidin also increased it. These results show that the plasma membrane potential of RBL 2H3 cells is set, in part, by the activity of the Na+ pump and in part by the K+ conductance, while that of rat mast cells is set predominantly by the Na+ pump.

• Authors: C Bronner, M Mousli, N Eleno, Y Landry
• Journal: FEBS letters (FEBS Lett) Vol. 255 Issue 2 Pg. 401-4 (Sep 25 1989) ISSN: 0014-5793 [Print] England
• PMID: 2477283 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Gramicidin
• Ouabain
• Sodium
• Potassium
• Calcium

Topics

• Animals
• Biological Transport, Active (drug effects)
• Calcium (pharmacology)
• Cell Line
• Cell Membrane (drug effects, physiology)
• Cells, Cultured
• Gramicidin (pharmacology)
• Kinetics
• Leukemia, Basophilic, Acute
• Male
• Mast Cells (physiology)
• Membrane Potentials (drug effects)
• Ouabain (pharmacology)
• Potassium (pharmacology)
• Rats
• Rats, Inbred Strains
• Sodium (metabolism)