Reperfusion of ischemic tissue leads to the generation of
oxygen derived
free radicals which plays an important role in cellular damage. Objective of the current study is to evaluate the cardio-protective and
antioxidant effect of
diosmin on
ischemia-reperfusion related cardiac dysfunction, oxidative stress and apoptosis.
Diosmin (50 and 100 mg/kg
body weight (bw)) was given every day to the rats orally throughout the experimental period.
Ischemia/reperfusion protocol was carried out ex vivo using langendorff perfusion method and the cardiac functional recovery was assessed in terms of percentage rate pressure product. Coronary effluents of LDH and CK-MB activities,
antioxidant enzyme activities, lipid peroxidation products, activity of TCA cycle
enzymes were evaluated. Moreover, in vitro
superoxide anion and
hydroxyl radical scavenging potential of
diosmin was also quantified. Finally, quantitative real-time PCR was used for assessing Bcl-2
mRNA expression in heart. Cardiac functional recovery was impaired after reperfusion compared with continuously perfused heart. It was significantly prevented by
diosmin treatment. Impaired
antioxidant enzyme activities and elevated lipid peroxidation products level were also significantly suppressed. The activity of TCA cycle
enzymes was protected against reperfusion stress. Down regulated Bcl-2 was also significantly increased. This study concluded that
diosmin pretreatment prevents all the impaired patterns including cardiac function, oxidative stress and apoptosis associated with reperfusion in control heart by its
antioxidant role.