Gene expression regulation of Bcl2, Bax and cytochrome-C by geraniol on chronic MPTP/probenecid induced C57BL/6 mice model of Parkinson's disease.

Abstract

Parkinson's disease (PD) is a common disabling movement disorder owing to progressive depletion of dopamine in nigrostriatal region, and can be experimentally accelerated by the neurotoxin 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP). MPTP-treated mice are a representative animal model for searching for the therapeutic agents for PD without adverse effect. In this study we investigated the effect of geraniol (GE) on chronic MPTP/probenecid (MPTP/p) induced apoptotic changes in nigrostriatal region. We observed that chronic exposure to MPTP/p led to increased expression of apoptotic markers, results in neurodegeneration and motor behavioral impairments in mice. Pretreatment with GE to MPTP/p significantly improved motor functions and ameliorated striatal antioxidant balance. In addition, GE attenuated the expression of apoptotic markers evident by the normalized Bcl-2/Bax ratio and decreased expression of cytochrome-C and caspase-9 in the substantia nigra and striatum of MPTP/p induced mice model of PD. The findings of the present study suggested that GE, a new therapeutic potential avenue may have beneficial effects in slowing or preventing the progression of PD and other neurodegenerative disorders.

• Authors: Karamkolly R Rekha, Govindasamy P Selvakumar
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 217 Pg. 57-66 (Jun 25 2014) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 24768735 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Acyclic Monoterpenes
• Proto-Oncogene Proteins c-bcl-2
• RNA, Messenger
• Terpenes
• bcl-2-Associated X Protein
• Cytochromes c
• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase
• geraniol
• Probenecid

Topics

• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (administration & dosage)
• Acyclic Monoterpenes
• Animals
• Apoptosis (physiology)
• Blotting, Western
• Catalase (blood)
• Cytochromes c (genetics, metabolism)
• Glutathione Peroxidase (blood)
• Immunohistochemistry
• Male
• Mice
• Mice, Inbred C57BL
• Motor Activity (drug effects)
• Parkinson Disease (blood, drug therapy, genetics, metabolism)
• Probenecid (administration & dosage)
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• RNA, Messenger (chemistry, genetics)
• Random Allocation
• Real-Time Polymerase Chain Reaction
• Superoxide Dismutase (blood)
• Terpenes (pharmacology)
• bcl-2-Associated X Protein (genetics, metabolism)