Abstract | BACKGROUND: METHODS: Sprague Dawley rats were randomized into control, model, Bufei Jianpi, Bufei Yishen, Yiqi Zishen and aminophylline groups. COPD rats, induced by cigarette smoke and bacterial infections exposures, were administrated intragastricly by normal saline, Bufei Jianpi, Bufei Yishen, Yiqi Zishen granules or aminophylline from week 9 through 20, respectively. At week 20 and 32, lung tissues were harvested. Immunohistochemistry was used to detect interleukin (IL)-1β and tumor necrosis factor (TNF)-α, quantitative real-time polymerase chain reaction (qRT-PCR) was used for TGF-β1 and Smad2 mRNA analysis, western blotting was used to determine the phosphorylation of NF-κB (p-NF-κB) and IκBα (p-IκBα). RESULTS:
COPD rats had marked airway injury, such as chronic airway inflammation and remodeling, emphysema, which were improved in the three traditional Chinese medicines (TCM)-treated animals. The levels of IL-1β, TNF-α, p-NF-κB, p-IκBα, TGF-β1 and Smad2 were significantly higher in COPD rats than in controls, while they were dramatically reduced in the three TCM- and aminophylline-treated groups. At the meantime, all these endpoints were significantly lower in three TCM-treated groups than in aminophylline group, especially in Bufei Jianpi and Bufei Yishen groups. Compared to week 20, all endpoints decreased significantly in three TCM groups at week 32. CONCLUSION: The three Tiao-Bu Fei-Shen therapies can reduce pulmonary inflammation and remodeling in COPD and have significant long-term effects. NF-κB/TGF-β1/smad2 signaling might be involved in the mechanism.
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Authors | Ya Li, Jian-sheng Li, Wei-wei Li, Su-yun Li, Yan-ge Tian, Xiao-fan Lu, Su-li Jiang, Ying Wang |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 14
Pg. 140
(Apr 26 2014)
ISSN: 1472-6882 [Electronic] England |
PMID | 24766819
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drugs, Chinese Herbal
- Interleukin-1beta
- NF-kappa B
- Smad2 Protein
- Transforming Growth Factor beta1
- Tumor Necrosis Factor-alpha
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Topics |
- Animals
- Disease Models, Animal
- Drugs, Chinese Herbal
(administration & dosage)
- Female
- Humans
- Interleukin-1beta
(metabolism)
- Lung
(drug effects, metabolism)
- Male
- NF-kappa B
(metabolism)
- Pulmonary Disease, Chronic Obstructive
(drug therapy, genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Signal Transduction
- Smad2 Protein
(genetics, metabolism)
- Time Factors
- Transforming Growth Factor beta1
(genetics, metabolism)
- Tumor Necrosis Factor-alpha
(genetics, metabolism)
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