Abstract | BACKGROUND: METHODS: RESULTS: Positive CCR7 expression in immunohistochemistory was detected in 28 (27%) of 105 consecutive patients and 17 (28%) of 61 T1 patients, which significantly correlated with lymph node metastasis (p = 0.037 and p = 0.040, respectively) and poor five-year survival (p = 0.013 and p = 0.012, respectively). Adhesion assay revealed an enhanced adhesive ability of CCR7-expressing cells in response to CCL21/SLC, in particular, in the presence of lymphatic endothelial cells (p = 0.005). In the mouse model, lymph nodes from mice transplanted with CCR7-expressing cells showed significantly higher DNA levels at 5 weeks (p = 0.019), indicating a high metastatic potential of CCR7-expressing cells. CONCLUSION: These results demonstrated the significant clinicopathological relationship and functional causality between CCR7 expression and lymph node metastasis in ESCC patients.
|
Authors | Tomoyuki Irino, Hiroya Takeuchi, Sachiko Matsuda, Yoshiro Saikawa, Hirofumi Kawakubo, Norihito Wada, Tsunehiro Takahashi, Rieko Nakamura, Kazumasa Fukuda, Tai Omori, Yuko Kitagawa |
Journal | BMC cancer
(BMC Cancer)
Vol. 14
Pg. 291
(Apr 26 2014)
ISSN: 1471-2407 [Electronic] England |
PMID | 24766770
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- CCL21 protein, human
- Chemokine CCL21
- Receptors, CCR7
|
Topics |
- Adult
- Aged
- Animals
- Carcinoma, Squamous Cell
(genetics, pathology)
- Chemokine CCL21
(biosynthesis, genetics)
- Esophageal Neoplasms
(genetics, pathology)
- Esophageal Squamous Cell Carcinoma
- Female
- Gene Expression Regulation, Neoplastic
(genetics)
- Humans
- Lymph Nodes
(metabolism, pathology)
- Lymphatic Metastasis
(genetics)
- Male
- Mice
- Middle Aged
- Receptors, CCR7
(biosynthesis, genetics)
- Transplantation, Heterotopic
|