Synergistic antitumoral effect of IL-12 gene cotransfected with antiangiogenic genes for angiostatin, endostatin, and saxatilin.

Abstract

Previously, it was reported that the cotransfection of angiostatin K1-3, endostatin, and saxatilin genes using cationic liposomes significantly inhibited tumor progression. IL-12 is a well-known immune modulator that promotes Th1-type antitumor immune responses and also induces antiangiogenic effects. In this study, we have examined the antitumoral function of the IL-12 gene cotransfected with antiangiogenic genes for angiostatin K1-3, endostatin, and saxatilin by O,O'-dimyristyl-N-lysyl glutamate (DMKE) cationic liposomes in a mouse tumor model. According to our results, the administration of the IL-12 gene or the genes for angiostatin K1-3, endostatin, and saxatilin exhibited effective inhibition of B16BL6 melanoma growth in mice. In particular, intravenous administration of the IL-12 gene along with intratumoral administration of the three antiangiogenic genes synergistically inhibited the B16BL6 tumor growth. These results suggest that systemically expressed IL-12 enhances antitumoral efficacy of locally expressed antiangiogenic proteins.

• Authors: Hong Sung Kim, Hwa Yeon Jeong, Yeon Kyung Lee, Keun Sik Kim, Yong Serk Park
• Journal: Oncology research (Oncol Res) Vol. 21 Issue 4 Pg. 209-16 ( 2013) ISSN: 1555-3906 [Electronic] United States
• PMID: 24762227 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Dipeptides
• Disintegrins
• Endostatins
• Liposomes
• O,O'-dimyristyl-N-lysyl glutamate
• saxatilin
• Interleukin-12
• Angiostatins
• DNA

Topics

• Angiostatins (biosynthesis, genetics)
• Animals
• Cell Growth Processes (genetics)
• DNA (administration & dosage, chemistry, genetics)
• Dipeptides (administration & dosage, chemistry)
• Disintegrins (biosynthesis, genetics)
• Endostatins (biosynthesis, genetics)
• Female
• Gene Expression
• Genetic Therapy (methods)
• Interleukin-12 (biosynthesis, genetics)
• Liposomes (administration & dosage, chemistry)
• Melanoma, Experimental (blood supply, genetics, pathology, therapy)
• Mice
• Mice, Inbred C57BL
• Plasmids (administration & dosage, chemistry, genetics)
• Transfection (methods)