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Immunohistochemical assessment of ATG7, LC3, and p62 in ameloblastomas.

AbstractBACKGROUND:
To investigate the roles of autophagy in tumorigenesis, cytodifferentiation, and prognosis of odontogenic tumors, we analyzed the immunohistochemical expression of ATG7, LC3, and p62 in odontogenic tissues.
METHODS:
Tissue specimens of nine dental follicles and 69 ameloblastomas were immunohistochemically examined with antibodies against ATG7, LC3, and p62.
RESULTS:
Immunohistochemical reactivity for ATG7, LC3, and p62 was detected in many odontogenic epithelial cells and several endothelial cells and fibroblasts in dental follicles and ameloblastomas. ATG7 reactivity in ameloblatomas was significantly higher than that in dental follicles. Expression of ATG7, LC3, and p62 was found markedly in neoplastic cells near the basement membrane rather than central polyhedral cells in ameloblastomas. Reactivity for these molecules was significantly higher in unicystic ameloblastomas than in solid ameloblastomas. Granular cells in granular cell ameloblastomas showed obvious reactivity for the autophagy- related molecules, and LC3 reactivity in granular cell ameloblastomas was significantly higher than in other ameloblastoma variations. Recurrent ameloblastomas showed significantly lower reactivity of LC3 and p62 than primary ameloblastomas.
CONCLUSIONS:
Expression of ATG7, LC3, and p62 in dental follicles and ameloblastomas suggests that autophagy regulation might be affected by microenvironment alterations during tumorigenesis. The molecular machinery for autophagy is possibly involved in tissue architecture, neoplastic cell differentiation, and prognosis of the benign epithelial odontogenic tumor.
AuthorsMiwa Okada, Mariko Oikawa, Yasuhiro Miki, Yoshinaka Shimizu, Seishi Echigo, Tetsu Takahashi, Hiroyuki Kumamoto
JournalJournal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 43 Issue 8 Pg. 606-12 (Sep 2014) ISSN: 1600-0714 [Electronic] Denmark
PMID24762217 (Publication Type: Journal Article)
Copyright© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Chemical References
  • Autoantigens
  • MAP1LC3A protein, human
  • Microtubule-Associated Proteins
  • P62 protein, human
  • RNA-Binding Proteins
  • Atg7 protein, human
  • Autophagy-Related Protein 7
  • Ubiquitin-Activating Enzymes
Topics
  • Adolescent
  • Adult
  • Ameloblastoma (chemistry, pathology)
  • Autoantigens (analysis)
  • Autophagy (physiology)
  • Autophagy-Related Protein 7
  • Basement Membrane (chemistry)
  • Carcinogenesis (chemistry, pathology)
  • Cell Differentiation (physiology)
  • Dental Sac (chemistry)
  • Endothelial Cells (chemistry)
  • Epithelial Cells (chemistry)
  • Female
  • Fibroblasts (chemistry)
  • Granular Cell Tumor (chemistry, pathology)
  • Humans
  • Immunohistochemistry
  • Male
  • Microtubule-Associated Proteins (analysis)
  • Middle Aged
  • Neoplasm Recurrence, Local (chemistry, pathology)
  • RNA-Binding Proteins (analysis)
  • Tumor Microenvironment (physiology)
  • Ubiquitin-Activating Enzymes (analysis)

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