[Antiproliferative effect of cycloartane-type triterpenoid from myrrh against human prostate cancer cells].

Abstract

OBJECTIVE:

To investigate the antiproliferative effect of cycloartan-24-ene-1alpha, 2alpha, 3beta-triol from Myrrh against human prostate cancer cells.

METHODS:

Morphological changes of compound-treated human prostate PC3 cells were determined by staining cells with Hoechst. The cell cycle progression and apoptosis were evaluated using flow cytometry assay. Apoptosis related protein levels were analyzed using Western blotting.

RESULTS:

Treatment of PC3 cells with cycloartan-24-ene-1alpha,2alpha,3beta-triol caused an increase ratio of apoptotic cells, which was verified by flow cytometry. It was also found that cycloartan-24-ene-1alpha,2alpha, 3beta-triol arrested cell cycle at the G0/G1 phase. Apoptosis related proteins, such as BCL-2, BAX, Caspase-3 and Caspase-9 were changed.

CONCLUSION:

These observes data indicate that cycloartan-24-ene-1alpha,2alpha,3beta-triol inhibit the proliferation of PC3 cells via induction of apoptosis and cell cycle arrest.

• Authors: Guo-Hui Li, Qing-Qing Zhong, Tao Shen
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 36 Issue 10 Pg. 1640-3 (Oct 2013) ISSN: 1001-4454 [Print] China
• PMID: 24761675 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• BAX protein, human
• Plant Exudates
• Proto-Oncogene Proteins c-bcl-2
• Triterpenes
• bcl-2-Associated X Protein
• CASP3 protein, human
• CASP9 protein, human
• Caspase 3
• Caspase 9

Topics

• Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Caspase 3 (metabolism)
• Caspase 9 (metabolism)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Commiphora (chemistry)
• Flow Cytometry
• Humans
• Male
• Molecular Structure
• Plant Exudates (chemistry, pharmacology)
• Prostatic Neoplasms (metabolism, pathology)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Triterpenes (chemistry, pharmacology)
• bcl-2-Associated X Protein (metabolism)