Expression of CD1 antigens by peripheral blood mononuclear cells from hepatitis B patients.

Abstract

We have studied the expression of CD1 antigens on peripheral blood mononuclear cells (PBMC) from acute hepatitis B patients in order to analyse a possible role for CD1 antigens in hepatitis B virus (HBV) infection. Using immunofluorescence and the monoclonal antibodies which recognized CD1a, CD1b and CD1c molecules, we have shown that CD1 antigens were expressed on PBMC from acute hepatitis B patients but not from other acute and chronic liver disease. Dot blot analysis on nitrocellulose sheets of the lysates of the cells confirmed these observations. Cell fractionation and double-labelling experiments clearly demonstrated the CD1 antigens were expressed only on non-T cells. Furthermore, CD1 antigens were coexpressed with hepatitis B surface antigen (HBsAg) on the surface of Ig-positive cells. These results could indicate that CD1 expression may be associated with the lymphotropic effect of HBV.

• Authors: F R Roisman, M C Salamone, A Mota, H Fainboim, L Fainboim
• Journal: Immunology letters (Immunol Lett) Vol. 21 Issue 2 Pg. 139-43 (May 1989) ISSN: 0165-2478 [Print] Netherlands
• PMID: 2475437 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD1
• Antigens, Differentiation
• Antigens, Surface

Topics

• Adolescent
• Adult
• Antibodies, Monoclonal
• Antigens, CD1
• Antigens, Differentiation (analysis)
• Antigens, Surface (immunology)
• Female
• Fluorescent Antibody Technique
• Hepatitis B (immunology)
• Humans
• Immunoblotting
• Leukocytes, Mononuclear (immunology)
• Male