HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Evidence for the metabolism of tumor promoter organic hydroperoxides into free radicals by human carcinoma skin keratinocytes: an ESR-spin trapping study.

Abstract
Humans are exposed to various peroxy and hydroperoxy compounds which are in use in the cosmetic, pharmaceutical and polymer industries and which are also generated as a result of the peroxidative metabolic conversion of certain lipids. This study was designed to determine whether the organic hydroperoxides, tert-butyl hydroperoxide, cumene hydroperoxide and tert-butyl peroxybenzoate are metabolized by human carcinoma skin keratinocytes to free radicals. Incubation of keratinocytes prepared from cutaneous squamous cell carcinoma in phosphate-buffered saline (pH 7.4) containing desferrioxamine with tert-butyl hydroperoxide, cumene hydroperoxide and tert-butyl peroxybenzoate in the presence of spin trap (3,5-dibromonitrosobenzene sulfonic acid) resulted in the generation of corresponding methyl radical adducts. Prior heating of the cells to 100 degrees C abolished the generation of radical adducts. The addition of ethanol to the reaction mixture also inhibited formation of radical adducts. These data provide the first direct evidence that human carcinoma skin cells can generate free radicals from organic hydroperoxides. Since free radicals are suggested to be involved in the cascade of events occurring during tumor promotion this metabolic capacity may be an important determinant of human cancer risk for hydroperoxides.
AuthorsM Athar, H Mukhtar, D R Bickers, I U Khan, B Kalyanaraman
JournalCarcinogenesis (Carcinogenesis) Vol. 10 Issue 8 Pg. 1499-503 (Aug 1989) ISSN: 0143-3334 [Print] England
PMID2473853 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Benzene Derivatives
  • Benzenesulfonates
  • Benzoates
  • Free Radicals
  • Nitroso Compounds
  • Peroxides
  • Spin Labels
  • tert-butyl peroxybenzoate
  • Keratins
  • 3,5-dibromo-4-nitrosobenzenesulfonate
  • tert-Butylhydroperoxide
  • cumene hydroperoxide
Topics
  • Benzene Derivatives (metabolism)
  • Benzenesulfonates
  • Benzoates (metabolism)
  • Carcinoma, Squamous Cell (metabolism)
  • Cell Line
  • Electron Spin Resonance Spectroscopy (methods)
  • Epidermal Cells
  • Epidermis (metabolism)
  • Free Radicals
  • Humans
  • Keratins (metabolism)
  • Kinetics
  • Nitroso Compounds
  • Peroxides (metabolism)
  • Skin Neoplasms (metabolism)
  • Spin Labels
  • tert-Butylhydroperoxide

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: