Abstract |
Corynoxeine(CX), isolated from the extract of Uncaria rhynchophylla, is a useful and prospective compound in the prevention and treatment for vascular diseases. A simple and selective liquid chromatography mass spectrometry (LC-MS) method was developed to determine the concentration of CX in rat plasma. The chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1% formic acid in water as mobile phase. Selective ion monitoring (SIM) mode was used for quantification using target ions m/z 383 for CX and m/z 237 for the carbamazepine (IS). After the LC-MS method was validated, it was applied to a back-propagation artificial neural network (BP-ANN) pharmacokinetic model study of CX in rats. The results showed that after intravenous administration of CX, it was mainly distributed in blood and eliminated quickly, t1/2 was less than 1h. The predicted concentrations generated by BP-ANN model had a high correlation coefficient (R>0.99) with experimental values. The developed BP-ANN pharmacokinetic model can be used to predict the concentration of CX in rats.
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Authors | Jianshe Ma, Jinzhang Cai, Guanyang Lin, Huilin Chen, Xianqin Wang, Xianchuan Wang, Lufeng Hu |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 959
Pg. 10-5
(May 15 2014)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 24732215
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Alkaloids
- Drugs, Chinese Herbal
- Indole Alkaloids
- corynoxeine
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Topics |
- Administration, Oral
- Alkaloids
(administration & dosage, pharmacokinetics)
- Animals
- Chromatography, High Pressure Liquid
(methods)
- Drugs, Chinese Herbal
(administration & dosage, pharmacokinetics)
- Indole Alkaloids
- Male
- Mass Spectrometry
(methods)
- Neural Networks, Computer
- Rats
- Rats, Sprague-Dawley
- Uncaria
(chemistry)
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