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Development of LC-MS determination method and back-propagation ANN pharmacokinetic model of corynoxeine in rat.

Abstract
Corynoxeine(CX), isolated from the extract of Uncaria rhynchophylla, is a useful and prospective compound in the prevention and treatment for vascular diseases. A simple and selective liquid chromatography mass spectrometry (LC-MS) method was developed to determine the concentration of CX in rat plasma. The chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1% formic acid in water as mobile phase. Selective ion monitoring (SIM) mode was used for quantification using target ions m/z 383 for CX and m/z 237 for the carbamazepine (IS). After the LC-MS method was validated, it was applied to a back-propagation artificial neural network (BP-ANN) pharmacokinetic model study of CX in rats. The results showed that after intravenous administration of CX, it was mainly distributed in blood and eliminated quickly, t1/2 was less than 1h. The predicted concentrations generated by BP-ANN model had a high correlation coefficient (R>0.99) with experimental values. The developed BP-ANN pharmacokinetic model can be used to predict the concentration of CX in rats.
AuthorsJianshe Ma, Jinzhang Cai, Guanyang Lin, Huilin Chen, Xianqin Wang, Xianchuan Wang, Lufeng Hu
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 959 Pg. 10-5 (May 15 2014) ISSN: 1873-376X [Electronic] Netherlands
PMID24732215 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier B.V. All rights reserved.
Chemical References
  • Alkaloids
  • Drugs, Chinese Herbal
  • Indole Alkaloids
  • corynoxeine
Topics
  • Administration, Oral
  • Alkaloids (administration & dosage, pharmacokinetics)
  • Animals
  • Chromatography, High Pressure Liquid (methods)
  • Drugs, Chinese Herbal (administration & dosage, pharmacokinetics)
  • Indole Alkaloids
  • Male
  • Mass Spectrometry (methods)
  • Neural Networks, Computer
  • Rats
  • Rats, Sprague-Dawley
  • Uncaria (chemistry)

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