Evidence for eosinophil degranulation with deposition of granule major basic protein in solar urticaria.

Abstract

Multiple sequential biopsy specimens of wheals elicited by a solar simulator in four patients with severe solar urticaria were studied with the use of indirect immunofluorescence for eosinophil granule major basic protein. Examination of control biopsy specimens from normal, unstimulated skin did not show eosinophils or major basic protein deposition. Five minutes after solar simulation, eosinophils were observed in vessels in the dermis, and 2 hours later there was marked tissue eosinophilia. Extracellular major basic protein was extensively deposited in the dermis at 2 and 24 hours, suggesting eosinophil degranulation. Thus evolution of the solar urticaria wheal is accompanied by infiltration of eosinophils and neutrophils and by tissue deposition of the eosinophil major basic protein, suggesting eosinophil degranulation.

• Authors: K M Leiferman, P G Norris, G M Murphy, J L Hawk, R K Winkelmann
• Journal: Journal of the American Academy of Dermatology (J Am Acad Dermatol) Vol. 21 Issue 1 Pg. 75-80 (Jul 1989) ISSN: 0190-9622 [Print] United States
• PMID: 2473098 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Blood Proteins
• Eosinophil Granule Proteins
• Ribonucleases

Topics

• Blood Proteins (metabolism)
• Cytoplasmic Granules (metabolism, ultrastructure)
• Eosinophil Granule Proteins
• Eosinophils (metabolism, pathology)
• Fluorescent Antibody Technique
• Humans
• Ribonucleases
• Skin (metabolism, pathology)
• Staining and Labeling
• Sunlight (adverse effects)
• Time Factors
• Urticaria (metabolism, pathology)