Effects of laser irradiation (670-nm InGaP and 830-nm GaAlAs) on burn of second-degree in rats.

Abstract

This study investigated the effects of 670-nm indium gallium phosphide (InGaP) and 830-nm gallium aluminum arsenide (GaAlAs) laser therapy on second-degree burns induced on the back of Wistar rats. Sixty-three male Wistar rats were anesthetized, and second-degree burns were made on their back. The animals were then divided randomly into three groups: control (C), animals treated with 670-nm InGaP laser (LIn), and animals treated with 830-nm GaAlAs laser (LGa). The wound areas were removed after 2, 6, 10, 14, and 18 days of treatment and submitted to structural and morphometric analysis. The following parameters were studied: total number of granulocytes and fibroblasts, number of newly formed blood vessels, and percentage of birefringent collagen fibers in the repair area. Morphometric analysis showed that different lasers 670-nm InGaP and 830-nm GaAlAs reduced the number of granulocytes and an increase of newly formed vessels in radiated lesions. The 670-nm InGaP laser therapy was more effective in increasing the number of fibroblasts. The different treatments modified the expression of VEGF and TGF-β1, when compared with lesions not irradiated. The different types of light sources showed similar effects, improved the healing of second-degree burns and can help for treating this type of injury. Despite the large number of studies with LLTI application in second-degree burns, there is still divergence about the best irradiation parameters to be used. Further studies are needed for developing a protocol effective in treating this type of injury.

• Authors: Gabriela Bortolança Chiarotto, Lia Mara Grosso Neves, Marcelo Augusto Marreto Esquisatto, Maria Esméria Corezola do Amaral, Gláucia Maria Tech dos Santos, Fernanda Aparecida Sampaio Mendonça
• Journal: Lasers in medical science (Lasers Med Sci) Vol. 29 Issue 5 Pg. 1685-93 (Sep 2014) ISSN: 1435-604X [Electronic] England
• PMID: 24729046 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Pancreatitis-Associated Proteins
• Phosphines
• REG3A protein, human
• Transforming Growth Factor beta1
• Vascular Endothelial Growth Factor A
• Indium
• Gallium
• indium phosphide

Topics

• Animals
• Birefringence
• Blood Vessels (pathology)
• Burns (pathology, therapy)
• Cell Count
• Fibroblasts (pathology)
• Gallium (chemistry)
• Indium (chemistry)
• Lasers, Semiconductor
• Low-Level Light Therapy
• Male
• Pancreatitis-Associated Proteins
• Phosphines (chemistry)
• Rats, Wistar
• Skin (pathology)
• Staining and Labeling
• Transforming Growth Factor beta1 (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)