Abstract | OBJECTIVE: the protective effects of a novel stilbene derivative, (e)-2-(3,4,5- trimethoxystyryl)-3,5,6-trimethylpyrazine (MStMp), on hydrogen peroxide (h2o2)-induced human derived neuroblastoma cell (Sh-Sy5y) damage and its molecular mechanisms were investigated. METHODS: RESULTS: MStMp increased the Sh-Sy5y cell viability by inhibition of cell apoptosis induced by h2o2. these effects were accompanied by an increase of Sod activity, gSh level, and a decrease of Mda content. Moreover, MStMp showed stronger effects on inhibition of ldh leakage, apoptotic cells, intracellular roS level and the expression of caspase-3 and caspase-9 than tMp. furthermore, MStMp induced a decrease of no level and the activity of inoS, tnoS in a time-dependent manner. CONCLUSIONS: MStMp prevents h2o2-induced cell injury through anti-oxidation and anti-apoptosis via roS-no pathway.
|
Authors | Wei-Guo Liu, Jun-Yi Zhao, Hao Zhang, Xin-Yong Liu, Xiu-Li Guo |
Journal | The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques
(Can J Neurol Sci)
Vol. 41
Issue 3
Pg. 382-8
(May 2014)
ISSN: 0317-1671 [Print] England |
PMID | 24718825
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Reactive Oxygen Species
- Stilbenes
- Hydrogen Peroxide
|
Topics |
- Apoptosis
(drug effects, physiology)
- Cell Line, Tumor
- Cell Survival
(drug effects, physiology)
- Dose-Response Relationship, Drug
- Humans
- Hydrogen Peroxide
(toxicity)
- Oxidative Stress
(drug effects, physiology)
- Reactive Oxygen Species
(antagonists & inhibitors, metabolism)
- Stilbenes
(chemistry, pharmacology)
|