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miR-22 inhibits proliferation and invasion in estrogen receptor α-positive endometrial endometrioid carcinomas cells.

Abstract
Endometrial endometrioid carcinomas (EECs) account for >80% of endometrial carcinomas (ECs). Continuous stimulation of the endometrium by estrogen is a risk factor for the tumorigenesis of estrogen receptor (ER) α-positive EEC. MicroRNA-22 (miR-22) has been reported to be implicated in the regulation of various types of cancer and directly targets ERα. However, an exact regulatory mechanism between miR-22 and ERα in EEC has yet to be investigated. To the best of our knowledge, the present study demonstrated for the first time that the expression of miR-22 was significantly downregulated in ERα-positive EEC tissues and cell lines, RL95-2 and Ishikawa, when compared with that in normal endometrium and ERα-negative EEC samples. This indicated that miR-22 may be important in ERα-positive EEC, possibly through an estrogen-dependent mechanism. miR-22 mimics were then transfected into RL95-2 and Ishikawa cells, respectively, and revealed that the introduction of miR-22 markedly downregulated the mRNA and protein levels of ERα. Further investigation demonstrated that miR-22 was able to effectively reverse 17β-estradiol (E2)‑induced cell proliferation, cell cycle progression and invasion of ERα-positive RL95-2 and Ishikawa cells, at least partially through inhibiting the expression of Cyclin D1 as well as the secretion of matrix metalloproteinase (MMP)-2 and MMP-9. In conclusion, the present study, to the best of our knowledge, was the first to reveal an inhibitory role of miR-22 in ERα-positive EEC tissues and cells, indicating that miR-22 may be a novel candidate for the endocrine therapy of ERα-positive EEC.
AuthorsShaoru Li, Ruili Hu, Chuanhong Wang, Fang Guo, Xiaoli Li, Shijin Wang
JournalMolecular medicine reports (Mol Med Rep) Vol. 9 Issue 6 Pg. 2393-9 (Jun 2014) ISSN: 1791-3004 [Electronic] Greece
PMID24715036 (Publication Type: Journal Article, Retracted Publication)
Chemical References
  • Estrogen Receptor alpha
  • MIRN22 microRNA, human
  • MicroRNAs
  • Cyclin D1
  • Estradiol
Topics
  • Carcinoma, Endometrioid (genetics, metabolism, pathology)
  • Cell Cycle (drug effects, genetics)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • Cyclin D1 (metabolism)
  • Estradiol (pharmacology)
  • Estrogen Receptor alpha (genetics, metabolism)
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs (genetics)
  • Neoplasm Invasiveness
  • Ovarian Neoplasms (genetics, metabolism, pathology)
  • Signal Transduction

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