Abstract | OBJECTIVE: To elucidate the mechanism by which embryo-resorption and preterm birth were enhanced by pathogenic CpG motif and to develop a counter strategy for normal pregnancy outcome. METHODS: This is an animal model-based study. In pregnant nonobese diabetic (NOD) mice and wild-type (WT) mice in the same strain background, an infection was mimicked by toll-like receptor 9 (TLR9) activation through CpG1826-injection. In vivo inactivation of IL-10 was performed to enhance pregnancy loss. Regulatory T cells induced by FTY720 in vitro from splenic CD4+CD25-Foxp3- cells (iTreg cells) were transferred to improve pregnancy outcomes in NOD mice. RESULTS: CONCLUSIONS: NOD mice are prone to abortion and preterm birth. This can be attributed to lacking Treg cells and insufficient IL-10 expression. Adoptive transfer of iTreg cells can rescue CpG-mediated pregnancy failure.
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Authors | Yi Lin, Xiaorui Liu, Bin Shan, Ji Wu, Surendra Sharma, Yun Sun |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 4
Pg. e94702
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24714634
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Forkhead Transcription Factors
- Foxp3 protein, mouse
- Oligodeoxyribonucleotides
- Interleukin-10
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Topics |
- Abortion, Spontaneous
(chemically induced, immunology, prevention & control)
- Adoptive Transfer
- Animals
- Disease Models, Animal
- Female
- Forkhead Transcription Factors
(metabolism)
- Immunophenotyping
- Interleukin-10
(metabolism)
- Lymphocyte Count
- Male
- Mice
- Mice, Inbred NOD
- Oligodeoxyribonucleotides
(administration & dosage, adverse effects)
- Phenotype
- Pregnancy
- Pregnancy Outcome
- Premature Birth
(chemically induced, immunology, prevention & control)
- T-Lymphocytes, Regulatory
(immunology, metabolism)
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