Vicenin 2 isolated from Artemisia capillaris exhibited potent anti-glycation properties.

Abstract

Vicenin 2, isolated from a traditionally used medicinal plant Artemisia capillaris, is a 6,8-di-C-glucoside of apigenin which has been previously reported to possess a wide variety of pharmacological activities including antioxidant, anti-inflammatory, anti-cancer, and hepatoprotective. However, there have not been any reports concerning its anti-diabetic potential until now. Therefore, in the present study, we evaluated the anti-diabetic potential of vicenin 2 via α-glucosidase, protein tyrosine phosphatase 1B (PTP1B), rat lens aldose reductase (RLAR), and advanced glycation end products (AGE) formation inhibitory assays. Vicenin 2 strongly inhibited α-glucosidase, PTP1B, and RLAR in the corresponding assays. In addition, vicenin 2 inhibited the formation of both fluorescent AGE and nonfluorescent AGE, e.g., CML, as well as the level of fructosamine in glucose-fructose-induced bovine serum albumin (BSA) glycation. In the test system, vicenin 2 suppressed glycation-induced protein oxidation by attenuating the formation of protein carbonyl groups as well as by inhibiting the modification of protein thiol groups. Moreover, vicenin 2 was found to be a potent inhibitor of glycation-induced formation of amyloid cross-β structures in BSA. Taken together, vicenin 2 might be a useful lead for the development of multiple target-oriented therapeutic modalities for the treatment of diabetes and diabetes-associated complications.

• Authors: Md Nurul Islam, Ishrat Jahan Ishita, Hyun Ah Jung, Jae Sue Choi
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 69 Pg. 55-62 (Jul 2014) ISSN: 1873-6351 [Electronic] England
• PMID: 24713265 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Amyloid
• Enzyme Inhibitors
• Glucosides
• Glycation End Products, Advanced
• Glycoside Hydrolase Inhibitors
• apigenin-6,8-di-C-glycopyranoside
• Serum Albumin, Bovine
• Fructosamine
• N(6)-carboxymethyllysine
• Apigenin
• Aldehyde Reductase
• Protein Tyrosine Phosphatase, Non-Receptor Type 1
• alpha-Glucosidases
• Lysine

Topics

• Aldehyde Reductase (antagonists & inhibitors, metabolism)
• Amyloid (chemistry, drug effects)
• Animals
• Apigenin (chemistry, isolation & purification, pharmacology)
• Artemisia (chemistry)
• Enzyme Inhibitors (pharmacology)
• Fructosamine (metabolism)
• Glucosides (chemistry, isolation & purification, pharmacology)
• Glycation End Products, Advanced (drug effects, metabolism)
• Glycoside Hydrolase Inhibitors (pharmacology)
• Lens, Crystalline (enzymology)
• Lysine (analogs & derivatives, metabolism)
• Oxidation-Reduction
• Plants, Medicinal (chemistry)
• Protein Carbonylation (drug effects)
• Protein Tyrosine Phosphatase, Non-Receptor Type 1 (antagonists & inhibitors, metabolism)
• Rats, Sprague-Dawley
• Serum Albumin, Bovine (chemistry, metabolism)
• alpha-Glucosidases (metabolism)