28 Beagle dogs were subjected to 50% total body surface area (TBSA)
burn, and they were divided into three groups:
burn injury without fluid
resuscitation (NR, n=8), Ringer
lactate solution (RL, n=10), and Pyr
Ringer solution (RP, n=10). They were given intravenous fluid
resuscitation according to Parkland formula 30 minutes after
burn. The hemodynamics, organ functions and mortality were observed in conscious state before
burn injury, and 2, 6, 8, 12, 24 hours after
burn injury.
RESULTS: Within 24 hours after
burn, all the dogs in NR group died, and those in RL and RP groups were all alive. At 2 hours after
burn, the mean arterial pressure (MAP), cardiac index (CI), dp/dt max of left ventricular contractility were significantly reduced in NR, RL and RP groups compared with those before injury (MAP: 45.33±7.78 mmHg vs. 141.67±5.98 mmHg, 91.33±10.25 mmHg vs. 142.33±6.16 mmHg, 98.67±9.54 mmHg vs. 142.83±5.47 mmHg; CI: 8.17±0.83 mL×s(-1)×m(-2) vs. 48.34±3.33 mL×s(-1)×m(-2), 16.84±2.17 mL×s(-1)×m(-2) vs. 47.34±1.67 mL×s(-1)×m(-2), 19.00±1.50 mL×s(-1)×m(-2) vs. 47.34±1.33 mL×s(-1)×m(-2); dp/dt max: 426.83±51.91 mmHg/s vs. 1 372.50±39.61 mmHg/s, 594.00±88.23 mmHg/s vs. 1 363.83±44.92 mmHg/s, 645.00±66.82 mmHg/s vs. 1 395.83±19.49 mmHg/s, all P<0.05), and the systemic vascular resistance (SVR) and
alanine transaminase (ALT),
creatinine (Cr), serum MB
isoenzyme of
creatine kinase (CK-MB),
diamine oxidase (DAO) were significantly higher (SVR: 1 322.50±36.37 kPa×s×L(-1) vs. 281.45±8.84 kPa×s×L(-1), 777.50±41.84 kPa×s×L(-1) vs. 289.72±6.70 kPa×s×L(-1), 571.40±40.01 kPa×s×L(-1) vs. 286.27±8.66 kPa×s×L(-1); ALT: 89.50±4.11 U/L vs. 40.57±3.63 U/L, 89.25±4.88 U/L vs. 37.92±2.62 U/
L, 86.30±5.61 U/L vs. 38.47±3.50 U/L; Cr: 75.62±4.61 μmol/L vs. 41.58±2.78 μmol/L, 77.00±5.92 μmol/L vs. 46.55±3.17 μmol/L, 74.13±2.56 μmol/L vs. 45.65±1.83 μmol/L; CK-MB: 13.122±0.282 kU/L vs. 1.557±0.009 kU/L, 8.885±0.272 kU/L vs. 1.497±0.009 kU/L, 8.692±0.180 kU/L vs. 1.490±0.005 kU/L; DAO: 2.26±0.14 kU/L vs. 0.25±0.02 kU/L, 1.50±0.07 kU/L vs. 0.25±0.01 kU/L, 1.37±0.07 kU/L vs. 0.25±0.02 kU/L, all P<0.05). All parameters in NR group kept on worsening till death, while hemodynamic and organ functions of two intravenous
resuscitation groups were gradually improved, CI, SVR and DAO in RP group were significantly superior to those of RL group from 2 hours on after
burn (all P<0.05), and dp/dt max and CK-MB in RP group were significantly better than those of RL group from 6 hours on after
burn (dp/dt max: 1,082.33±63.59 mmHg/s vs. 1,018.60±47.36 mmHg/s, CK-MB: 7,898.70±255.74 U/L vs. 8,438.70±442.00 U/L, all P<0.05), and MAP was significantly better than that of RL group at 6 hours (124.67±9.39 mmHg vs. 114.33±9.16 mmHg, P<0.05), and Cr was significantly better than that of RL group from 24 hours on after
burn (53.42±4.99 μmol/L vs. 60.77±3.11 μmol/L, P<0.05).
CONCLUSIONS: