Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: MATERIALS AND METHODS: Rats were divided into 9 groups, and drugs were administered from on the day after the onset of arthritis (day 12) until day 31 of the experiment once daily continuously. Urine and plasma were analyzed by reversed-phase liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). Partial least-squares discriminate analysis (PLS-DA) models were built to evaluate the therapeutic effects of HLJDT and its constituents combination. 15 identified CIA biomarkers were investigated to explain its therapeutic mechanism. RESULTS: Administration of HLJDT and its constituents combination in CIA rats not only significantly reduced arthritic scores and serum levels of IL-1β but also improved histopathologic changes in joint architecture. Urinary and plasma metabolic profiling revealed that perturbation of energy metabolism, lipid metabolism, oxidative injury and some amino acids metabolism occurred in collagen-induced arthritis (CIA). Our results also indicated that the disturbed urinary levels of succinic acid, citric acid, creatine, uridine, pantothenic acid, carnitine, phenylacetylglycine, allantoin and plasma levels of phenylpyruvic acid in model rats were gradually restored to normal after administration of HLJDT. The treatment of constituents combination of HLJDT group was able to restore to normal the disturbed urinary levels of citric acid, creatine, pantothenic acid, carnitine, pantothenic acid, phenylacetylglycine and plasma levels of uric acid, L-histidine, and l-phenylalanine in model rats. CONCLUSIONS: Our study indicates that HLJDT and its constituents combination treatment can ameliorate CIA through partially regulating the perturbed energy metabolism. Our work demonstrated that metabonomics-based approach is a promising new tool to evaluate the therapeutic effects and mechanism of complex TCM prescriptions.
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Authors | Huawei Zhang, Peng Fu, Beilei Ke, Shuping Wang, Min Li, Lin Han, Chengcheng Peng, Weidong Zhang, Runhui Liu |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 154
Issue 1
Pg. 55-64
(May 28 2014)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 24709313
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Biomarkers
- Drugs, Chinese Herbal
- Interleukin-1beta
- huang-lien-chieh-tu-tang
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology, therapeutic use)
- Arthritis, Experimental
(blood, drug therapy, pathology, urine)
- Arthritis, Rheumatoid
(blood, drug therapy, pathology, urine)
- Biomarkers
(blood, urine)
- Drugs, Chinese Herbal
(pharmacology, therapeutic use)
- Energy Metabolism
(drug effects)
- Interleukin-1beta
(blood)
- Knee Joint
(drug effects, pathology)
- Male
- Metabolomics
- Rats, Wistar
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