Strong delayed-type hypersensitivity induced against house dust mite antigens in the mice.

Abstract

The aim of this study was to assess the antigenicity of house dust mite antigens using delayed-type hypersensitivity (DTH) responses in mice. The crude extracts of Dermatophagoides pteronyssinus and Dermatophagoides farinae induced strong DTH responses and was transferred with T cells. Assessment of DTH responses to the house dust revealed that substantial cross-reactivity at T-cell level was observed between these two mite extracts. Almost all the fractions of the extract of D. pteronyssinus, which has a molecular weight of between 10 and 100 kilodaltons, elicited strong DTH responses. It was found that antigenicity of the mite extract was reduced by chemical denaturation with tannic acid.

• Authors: Y Nakano, M Yoshida, T Shibata
• Journal: International archives of allergy and applied immunology (Int Arch Allergy Appl Immunol) Vol. 88 Issue 4 Pg. 434-8 ( 1989) ISSN: 0020-5915 [Print] Switzerland
• PMID: 2470689 (Publication Type: Journal Article)

Chemical References

• Allergens
• Antigens, Dermatophagoides
• Epitopes
• Hydrolyzable Tannins
• Cyclophosphamide

Topics

• Allergens (immunology)
• Animals
• Antigens, Dermatophagoides
• Chromatography, Gel
• Cyclophosphamide (pharmacology)
• Epitopes
• Female
• Hydrolyzable Tannins
• Hypersensitivity, Delayed (chemically induced, immunology)
• Immunization, Passive
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Inbred ICR
• Mites (immunology)