Molecular cloning of a gene encoding a Chlamydia psittaci 57-kDa protein that shares antigenic determinants with ca. 60-kDa proteins present in many gram-negative bacteria.

Abstract

In order to develop reagents to study the immune response of guinea pigs to infection by Chlamydia psittaci guinea pig inclusion conjunctivitis strain (GPIC), we constructed a plasmid clone bank with C. psittaci DNA. One of the recombinant clones isolated produced large amounts of a 57-kilodalton (kDa) protein that was immunoreactive with sera from GPIC infected guinea pigs. While investigating this recombinant protein, we discovered that all the Gram-negative bacteria analyzed so far have immunoreactive proteins of similar size. This protein seems to be a 'common antigen' already described in various Gram-negative bacteria.

• Authors: F D Menozzi, C Menozzi-Dejaiffe, F E Nano
• Journal: FEMS microbiology letters (FEMS Microbiol Lett) Vol. 49 Issue 1 Pg. 59-63 (Mar 1989) ISSN: 0378-1097 [Print] England
• PMID: 2470639 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• Epitopes

Topics

• Antigens, Bacterial (genetics, immunology)
• Bacterial Proteins (genetics, immunology)
• Chlamydophila psittaci (genetics)
• Cloning, Molecular
• Epitopes (analysis)
• Gram-Negative Bacteria (immunology)
• Staphylococcus (immunology)
• Yeasts (immunology)