Glutamine protects the small intestinal mucosa in anticancer drug-induced rat enteritis model.

Abstract

Supportive therapy during chemotherapy has become essential, but effective preventive therapies to gastrointestinal mucosal injury are few. We investigated the efficacy of glutamine in rat anticancer drug-induced enteritis model. In this study, we used twenty male SD rats. They were divided into control, 5-fluorouracil (5-FU) (orally administered at 20 mg/kg day), 5-FU+glutamine (1000 mg/kg/day) and 5-FU+glutamine+fiber and oligosaccharide (GFO(®)) (1000 mg/kg/day) groups. All groups were sacrificed on day 6 and upper jejunums were excised. The jejunal villous height was measured in specimens. IgA level in jejunal washing solution, and serum diamine oxidase activity were also measured. The jejunal villous height was recognized as shorter in the specimen from 5-FU treated rats compared with 5-FU+glutamine treated rats (p<0.001). Serum diamine oxidase activity in 5-FU+glutamine group were significantly superior to that in 5-FU group (p=0.028). IgA level in jejunal washing solution tended to be higher in 5-FU+glutamine group than that in 5-FU group (p=0.076). On the other hand, serum diamine oxidase activity and IgA level in jejunal washing solution showed no significant difference between 5-FU+GFO and 5-FU treatment group. Our results suggest that glutamine showed protective effects on mucosal injury of small intestine in rat anticancer drug-induced enteritis model.

• Authors: Hirokazu Takechi, Kazuaki Mawatari, Nagakatsu Harada, Yutaka Nakaya, Megumi Asakura, Mutsumi Aihara, Hiromitsu Takizawa, Masakazu Goto, Takeshi Nishino, Takuya Minato, Yoshihito Furukita, Yota Yamamoto, Yasuhiro Yuasa, Hiromichi Yamai, Takahiro Yoshida, Junichi Seike, Akira Tangoku
• Journal: The journal of medical investigation : JMI (J Med Invest) Vol. 61 Issue 1-2 Pg. 59-64 ( 2014) ISSN: 1349-6867 [Electronic] Japan
• PMID: 24705750 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Dietary Fiber
• Immunoglobulin A
• Oligosaccharides
• Glutamine
• Amine Oxidase (Copper-Containing)
• Fluorouracil

Topics

• Administration, Oral
• Amine Oxidase (Copper-Containing) (blood)
• Animals
• Antineoplastic Agents (administration & dosage, adverse effects, pharmacology)
• Dietary Fiber (administration & dosage, pharmacology)
• Disease Models, Animal
• Enteritis (chemically induced, pathology, prevention & control)
• Fluorouracil (administration & dosage, adverse effects, pharmacology)
• Glutamine (administration & dosage, pharmacology, therapeutic use)
• Immunoglobulin A (blood)
• Intestinal Mucosa (drug effects, metabolism, pathology)
• Jejunum (drug effects, metabolism, pathology)
• Male
• Oligosaccharides (administration & dosage, pharmacology)
• Rats
• Rats, Sprague-Dawley