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(-)-Epigallocatechin-3-gallate ameliorates photodynamic therapy responses in an in vitro T lymphocyte model.

Abstract
(-)-Epigallocatechin-3-gallate (EGCG), the most abundant polyphenolic constituent in green tea, is known as a powerful antioxidant but concomitantly possesses a prooxidant property. We investigated the effect of EGCG on phloxine B (PhB)-induced photocytotoxicity in human T lymphocytic leukemia Jurkat cells. EGCG significantly potentiated PhB-induced photocytotoxic effects, including the inhibition of cell proliferation, DNA fragmentation, and caspase-3 activity induction in Jurkat cells. Catalase attenuated the enhanced cytotoxicity by EGCG, suggesting the involvement of extracellularly produced hydrogen peroxide. Indeed, EGCG significantly enhanced extracellular hydrogen peroxide formation induced by photo-irradiated PhB. The EGCG also enhanced intracellular reactive oxygen species accumulation, c-Jun N-terminal kinase (JNK) phosphorylation, and interferon-γ (IFN-γ) gene expression, all of which are involved in PhB-induced apoptosis. Taken together, our data suggest that EGCG is capable of potentiating photodynamic therapy responses, presumably through the intracellular oxidative stress-sensitive JNK/IFN-γ pathway by exogenous hydrogen peroxide formation.
AuthorsHang Qi, Naomi Abe, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura
JournalPhytotherapy research : PTR (Phytother Res) Vol. 28 Issue 10 Pg. 1486-91 (Oct 2014) ISSN: 1099-1573 [Electronic] England
PMID24700514 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 John Wiley & Sons, Ltd.
Chemical References
  • PHB protein, human
  • Prohibitins
  • Reactive Oxygen Species
  • Interferon-gamma
  • Catechin
  • Hydrogen Peroxide
  • epigallocatechin gallate
  • Catalase
  • JNK Mitogen-Activated Protein Kinases
  • CASP3 protein, human
  • Caspase 3
Topics
  • Apoptosis (drug effects)
  • Caspase 3 (metabolism)
  • Catalase (metabolism)
  • Catechin (analogs & derivatives, pharmacology)
  • Cell Proliferation (drug effects)
  • DNA Fragmentation
  • Humans
  • Hydrogen Peroxide (metabolism)
  • Interferon-gamma (metabolism)
  • JNK Mitogen-Activated Protein Kinases (metabolism)
  • Jurkat Cells (drug effects)
  • Photochemotherapy
  • Prohibitins
  • Reactive Oxygen Species (metabolism)

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