Abstract |
Two-hundred MRSA strains from inpatients with healthcare-associated (HA) and 100 MRSA strains from outpatients with community-associated (CA) skin and soft tissue infections (SSTIs) were tested for antimicrobial susceptibility, staphylococcal cassette chromosome mec (SCCmec) typing, Panton-Valentine leucocidin (PVL) toxin, seh and arcA genes. Based on SCCmec typing, HA-MRSA isolates were further divided into HA-SCCmec I/II/III MRSA and HA-SCCmec IV/V MRSA, and CA-MRSA isolates into CA-SCCmec I/II/III MRSA and CA-SCCmec IV/V MRSA. SCCmec types were further characterized by pulsed-field gel electrophoresis, spa typing and multi-locus sequence typing. Seventy-five (37·5%) HA-MRSA isolates and 83/100 CA-MRSA isolates were SCCmec IV/V genotype. HA-SCCmec IV/V MRSA was associated with malignancy (P = 0·03) and bone fractures (P = 0·02) compared to CA-SCCmec IV/V MRSA. HA-SCCmec IV/V MRSA was associated with PVL gene carriage compared to HA-SCCmec I/II/III MRSA (P < 0·001). ST22-MRSA-IV (EMRSA-15), ST772-MRSA-V, and ST36-MRSA-IV and ST239:EMRSA-I:III were the major clones identified. Our study documents the emergence of SCCmec IV and SCCmec V MRSA clones in an Indian hospital.
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Authors | B Dhawan, C Rao, E E Udo, R Gadepalli, S Vishnubhatla, A Kapil |
Journal | Epidemiology and infection
(Epidemiol Infect)
Vol. 143
Issue 2
Pg. 343-53
(Jan 2015)
ISSN: 1469-4409 [Electronic] England |
PMID | 24690229
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Bacterial Agents
- Bacterial Proteins
- Bacterial Toxins
- Enterotoxins
- Exotoxins
- Leukocidins
- Panton-Valentine leukocidin
- Staphylococcal enterotoxin H
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Topics |
- Adolescent
- Adult
- Anti-Bacterial Agents
(pharmacology)
- Bacterial Proteins
(genetics)
- Bacterial Toxins
(genetics)
- Child
- Cross Infection
(epidemiology, microbiology, prevention & control)
- Enterotoxins
(genetics)
- Exotoxins
(genetics)
- Female
- Humans
- Infection Control
- Leukocidins
(genetics)
- Male
- Methicillin Resistance
(genetics)
- Methicillin-Resistant Staphylococcus aureus
(classification, drug effects, genetics, isolation & purification)
- Microbial Sensitivity Tests
- Middle Aged
- Multilocus Sequence Typing
- Staphylococcal Infections
(epidemiology, microbiology, prevention & control)
- Tertiary Care Centers
(statistics & numerical data)
- Young Adult
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