Cytotoxic T lymphocyte recognition of secreted HLA class I molecules.

Abstract

The cytolytic responses of DBA/2 mice against syngeneic transfected P815 mastocytoma cells expressing either membrane-associated (HLA-Cw3) or -secreted hybrid (HLA-Cw3 x H-2 Q10b) molecules were compared. In spite of the absence of serologically detectable hybrid molecules on their plasma membrane, cells secreting these molecules elicited a CTL response similar to that of cells expressing the membrane associated HLA-Cw3 molecules, in terms of both MHC-restriction and peptide specificity. Together with the observation that syngeneic mice were capable of rejecting the injected secreting cells, these results imply that secreted HLA class I molecules can function as minor histocompatibility Ag and suggest that processing of both the membrane-bound and the -secreted forms of a protein may follow common or overlapping pathways.

• Authors: B Kahn-Perles, C Barra, J Jehan, D Fourel, M Barad, J L Maryanski, F A Lemonnier
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 142 Issue 9 Pg. 3021-5 (May 01 1989) ISSN: 0022-1767 [Print] United States
• PMID: 2468709 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Epitopes
• HLA Antigens
• HLA-C Antigens
• HLA-C*03 antigen

Topics

• Animals
• Cell Line
• Clone Cells (immunology, transplantation)
• Epitopes (genetics)
• Genes, Synthetic
• HLA Antigens (analysis, genetics, metabolism)
• HLA-C Antigens (genetics)
• Humans
• Male
• Mast-Cell Sarcoma (metabolism)
• Mice
• Mice, Inbred DBA
• T-Lymphocytes, Cytotoxic (immunology, transplantation)
• Transfection