Gastroprotective effect of epoxy clerodane diterpene isolated from Tinospora cordifolia Miers (Guduchi) on indomethacin-induced gastric ulcer in rats.

Abstract

The present study evaluated the gastroprotective effect of epoxy clerodane diterpene (ECD), isolated from Tinospora cordifolia on indomethacin-induced gastric ulcer in rats. Administration of indomethacin exhibits extreme levels of ulcer index (UI) and myeloperoxidase (MPO) activity. Indomethacin down regulated PGE2, anti-inflammatory cytokines (IL-4, IL-10) and pro-angiogenic factors (VEGF and EGF). The ECD pretreatment considerably increased the levels of PGE2, anti-inflammatory cytokines and pro-angiogenic factors. The ulcer-healing activity of ECD was inhibited by pre-administration of the specific COX-1 inhibitor (SC560) and nonspecific NOS inhibitor (L-NAME), which indicates the involvement of PGE2 and NOS in ECD induced ulcer healing activity. These findings suggest that ECD exerts its antiulcer activity by reinforcement of defensive elements and diminishing the offensive elements.

• Authors: Paulrayer Antonisamy, Muniyappan Dhanasekaran, Savarimuthu Ignacimuthu, Veeramuthu Duraipandiyan, Joseph Devadass Balthazar, Paul Agastian, Jong-Hoon Kim
• Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (Phytomedicine) Vol. 21 Issue 7 Pg. 966-9 (Jun 15 2014) ISSN: 1618-095X [Electronic] Germany
• PMID: 24680619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier GmbH. All rights reserved.

Chemical References

• (5R,10R)-4R,8R-dihydroxy-2S,3R:15,16-diepoxycleroda-13(16),17,12S:18,1S-dilactone
• Cyclooxygenase Inhibitors
• Cytokines
• Diterpenes, Clerodane
• Enzyme Inhibitors
• Protective Agents
• Pyrazoles
• SC 560
• Dinoprostone
• NG-Nitroarginine Methyl Ester
• Indomethacin

Topics

• Animals
• Cyclooxygenase Inhibitors (pharmacology)
• Cytokines (metabolism)
• Dinoprostone (metabolism)
• Diterpenes, Clerodane (isolation & purification, pharmacology, toxicity)
• Enzyme Inhibitors (pharmacology)
• Female
• Gastric Mucosa (drug effects, pathology)
• Indomethacin (adverse effects)
• Male
• NG-Nitroarginine Methyl Ester (pharmacology)
• Protective Agents (pharmacology)
• Pyrazoles (pharmacology)
• Rats, Wistar
• Stomach Ulcer (chemically induced, drug therapy, pathology)
• Tinospora (chemistry)
• Toxicity Tests, Acute