Abstract |
In normal tissues aspartyl tRNA is present in 3 separable components by RPC-5-chromatography, 2 small and early eluting peaks and a major component designated rRNAAspIII. A late eluting peak, tRNAAspIV has also been found in many tumors of human and animals origin, and in BHK 21/13 cells grown in culture, where 27% or more of the total aspartate acceptance occurs in this peak. In contrast, normal tissue studied, i.e., hamster liver or kidney, exhibits less than 2.0% of the total aspartyl tRNA as the late eluting tRNAAspIV. Increasing the level of fetal calf serum in the medium from 5 to 15% resulted in a change from 0.79 to 1.83 in the ratio of tRNAAspIII--tRNAAspIV in BHK cells. Utilizing sera from different species, the percentage of tRNAAspIV was found to vary between 26.8 and 66.0% while the combined percentages of tRNAAspIII and tRNAAspIV varied between 59.5 and 80.8%. It appears that the increased tRNAAspIII was derived from tRNAAspIV and vice versa indicating that there may be a close structural relationship between tRNAAspIII and tRNAAspIV. It was also observed that BHK tumor cells or ascitic fluid enhanced the appearance of tRNAAspIV in normal host liver.
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Authors | M V Marshall, D L Adams, A C Griffin |
Journal | Cancer letters
(Cancer Lett)
Vol. 4
Issue 3
Pg. 147-53
(Mar 1978)
ISSN: 0304-3835 [Print] Ireland |
PMID | 246753
(Publication Type: Journal Article)
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Chemical References |
- Culture Media
- RNA, Neoplasm
- RNA, Transfer, Amino Acyl
- Aspartic Acid
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Topics |
- Animals
- Aspartic Acid
(metabolism)
- Cells, Cultured
- Chromatography
- Culture Media
- Kidney
(metabolism)
- Liver
(metabolism)
- Mice
- Neoplasms, Experimental
(metabolism)
- RNA, Neoplasm
(isolation & purification, metabolism)
- RNA, Transfer, Amino Acyl
(isolation & purification, metabolism)
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