Abstract | BACKGROUND: METHODS: An MTT assay was performed involving the treatment of MCF-7 cells for 48 h with H2O2 alone and H2O2 + different asiaticoside concentrations. Flow cytometry was performed, and the level of caspase 3, tumour necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1) were quantified. Adult female Sprague-Dawley (SD) rats were divided into five groups designated I (control), II (DMBA-induced cancer), III (pre- and post-treatment with asiaticoside (200 μg/animal) in DMBA-induced cancer), IV (post-treatment with asiaticoside in DMBA-induced cancer), and V (treated with asiaticoside alone, drug control). Twelve weeks post-DMBA, rats developed mammary tumours. Rats either were sacrificed or imaged with MIBI. Histological examination of tumour tissues was performed. Tumour MIBI uptake ratios were determined. The data are expressed as the means ± standard deviation. Appropriate t-test and ANOVA statistical methods were used to compare data. RESULTS: CONCLUSION: This study demonstrates that asiaticoside is effective in vitro and in vivo in inducing apoptosis and enhancing anti-tumour activity.
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Authors | Fatma J Al-Saeedi |
Journal | BMC cancer
(BMC Cancer)
Vol. 14
Pg. 220
(Mar 25 2014)
ISSN: 1471-2407 [Electronic] England |
PMID | 24667059
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Biomarkers, Tumor
- Triterpenes
- Hydrogen Peroxide
- asiaticoside
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Topics |
- Animals
- Antineoplastic Agents
(administration & dosage, toxicity)
- Apoptosis
(drug effects)
- Biomarkers, Tumor
(metabolism)
- Breast Neoplasms
(diagnostic imaging, drug therapy, pathology)
- Cell Line, Tumor
- DNA Damage
(drug effects)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Hydrogen Peroxide
(pharmacology)
- MCF-7 Cells
- Mammary Neoplasms, Experimental
- Microscopy, Electron, Scanning
- Radionuclide Imaging
- Rats
- Rats, Sprague-Dawley
- Triterpenes
(administration & dosage, toxicity)
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