HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

In vivo imaging of mGluR5 changes during epileptogenesis using [11C]ABP688 PET in pilocarpine-induced epilepsy rat model.

AbstractINTRODUCTION:
Metabotropic glutamate receptor 5 (mGluR5) that regulates glutamatergic neurotransmission contributes to pathophysiology of epilepsy. In this study, we monitored the changes of mGluR5 in vivo using [11C]ABP688 PET during the epileptogenesis in a pilocarpine-induced epilepsy rat model.
METHODS:
In vivo mGluR5 images were acquired using [11C]ABP688 microPET/CT in pilocarpine-induced chronic epilepsy rat models and controls. We also acquired microPET/CT at acute, subacute as well as chronic periods after status epilepticus. Non-displaceable binding potential (BPND) of [11C]ABP688 was calculated using simplified reference tissue model in a voxel-based manner. mGluR5 BPND of the rat brains of epilepsy models and controls were compared.
RESULTS:
Status epilepticus developed after pilocarpine administration and was followed by recurrent spontaneous seizures for more than 3 weeks. In chronic epilepsy rat model, BPND in hippocampus and amygdala was reduced on a voxel-based analysis. Temporal changes of mGluR5 BPND was also found. In acute period after status epilepticus, mGluR5 BPND was reduced in the whole brain. BPND of caudate-putamen was restored in subacute period, while BPND of the rest of the brain was still lower. In chronic period, global BPND was normalized except in hippocampus and amygdala.
CONCLUSIONS:
In vivo imaging of mGluR5 using [11C]ABP688 microPET/CT could successfully reveal the regional changes of mGluR5 binding potential of the rat brain in a pilocarpine-induced epilepsy model. The temporal and spatial changes in mGluR5 availability suggest [11C]ABP688 PET imaging in epilepsy provide abnormal glutamatergic network during epileptogenesis.
AuthorsHongyoon Choi, Yu Kyeong Kim, So Won Oh, Hyung-Jun Im, Do Won Hwang, Hyejin Kang, Boeun Lee, Yun-Sang Lee, Jae Min Jeong, E Edmund Kim, June-Key Chung, Dong Soo Lee
JournalPloS one (PLoS One) Vol. 9 Issue 3 Pg. e92765 ( 2014) ISSN: 1932-6203 [Electronic] United States
PMID24663806 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 3-(6-methylpyridin-2-ylethynyl)cyclohex-2-enone-O-methyloxime
  • Grm5 protein, rat
  • Muscarinic Agonists
  • Oximes
  • Pyridines
  • Receptor, Metabotropic Glutamate 5
  • Pilocarpine
Topics
  • Amygdala (diagnostic imaging, metabolism, physiopathology)
  • Animals
  • Epilepsy (chemically induced, diagnostic imaging, metabolism, physiopathology)
  • Hippocampus (diagnostic imaging, metabolism, physiopathology)
  • Male
  • Muscarinic Agonists (adverse effects, pharmacology)
  • Oximes (pharmacology)
  • Pilocarpine (adverse effects, pharmacology)
  • Positron-Emission Tomography
  • Pyridines (pharmacology)
  • Radiography
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Metabotropic Glutamate 5 (metabolism)
  • Synaptic Transmission (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: