Abstract | INTRODUCTION: METHODS: In vivo mGluR5 images were acquired using [11C] ABP688 microPET/CT in pilocarpine-induced chronic epilepsy rat models and controls. We also acquired microPET/CT at acute, subacute as well as chronic periods after status epilepticus. Non-displaceable binding potential (BPND) of [11C] ABP688 was calculated using simplified reference tissue model in a voxel-based manner. mGluR5 BPND of the rat brains of epilepsy models and controls were compared. RESULTS:
Status epilepticus developed after pilocarpine administration and was followed by recurrent spontaneous seizures for more than 3 weeks. In chronic epilepsy rat model, BPND in hippocampus and amygdala was reduced on a voxel-based analysis. Temporal changes of mGluR5 BPND was also found. In acute period after status epilepticus, mGluR5 BPND was reduced in the whole brain. BPND of caudate-putamen was restored in subacute period, while BPND of the rest of the brain was still lower. In chronic period, global BPND was normalized except in hippocampus and amygdala. CONCLUSIONS: In vivo imaging of mGluR5 using [11C] ABP688 microPET/CT could successfully reveal the regional changes of mGluR5 binding potential of the rat brain in a pilocarpine-induced epilepsy model. The temporal and spatial changes in mGluR5 availability suggest [11C] ABP688 PET imaging in epilepsy provide abnormal glutamatergic network during epileptogenesis.
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Authors | Hongyoon Choi, Yu Kyeong Kim, So Won Oh, Hyung-Jun Im, Do Won Hwang, Hyejin Kang, Boeun Lee, Yun-Sang Lee, Jae Min Jeong, E Edmund Kim, June-Key Chung, Dong Soo Lee |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 3
Pg. e92765
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24663806
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3-(6-methylpyridin-2-ylethynyl)cyclohex-2-enone-O-methyloxime
- Grm5 protein, rat
- Muscarinic Agonists
- Oximes
- Pyridines
- Receptor, Metabotropic Glutamate 5
- Pilocarpine
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Topics |
- Amygdala
(diagnostic imaging, metabolism, physiopathology)
- Animals
- Epilepsy
(chemically induced, diagnostic imaging, metabolism, physiopathology)
- Hippocampus
(diagnostic imaging, metabolism, physiopathology)
- Male
- Muscarinic Agonists
(adverse effects, pharmacology)
- Oximes
(pharmacology)
- Pilocarpine
(adverse effects, pharmacology)
- Positron-Emission Tomography
- Pyridines
(pharmacology)
- Radiography
- Rats
- Rats, Sprague-Dawley
- Receptor, Metabotropic Glutamate 5
(metabolism)
- Synaptic Transmission
(drug effects)
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