Abstract |
Branched RNA-linked multicopy single-stranded DNA (msDNA) originally detected in myxobacteria has now been found in a clinical isolate of Escherichia coli. Although lacking homology in the primary structure, the E. coli msDNA is similar in secondary structure to the myxobacterial msDNA's, including the 2',5'-phosphodiester linkage between RNA and DNA. A chromosomal DNA fragment responsible for the production of msDNA was cloned in an E. coli K12 strain; its DNA sequence revealed an open reading frame (ORF) of 586 amino acid residues. The ORF shows sequence similarity with retroviral reverse transcriptases and ribonuclease H. Disruption of the ORF blocked msDNA production, indicating that this gene is essential for msDNA synthesis.
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Authors | B C Lampson, J Sun, M Y Hsu, J Vallejo-Ramirez, S Inouye, M Inouye |
Journal | Science (New York, N.Y.)
(Science)
Vol. 243
Issue 4894 Pt 1
Pg. 1033-8
(Feb 24 1989)
ISSN: 0036-8075 [Print] United States |
PMID | 2466332
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA Probes
- DNA, Bacterial
- DNA, Single-Stranded
- RNA, Bacterial
- msDNA
- RNA-Directed DNA Polymerase
- Endoribonucleases
- DNA Restriction Enzymes
- Ribonuclease H
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Topics |
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Probes
- DNA Restriction Enzymes
- DNA, Bacterial
(genetics)
- DNA, Single-Stranded
(analysis, biosynthesis, genetics)
- Endoribonucleases
(genetics)
- Escherichia coli
(enzymology, genetics)
- Genes, Bacterial
- HIV
(enzymology, genetics)
- Human T-lymphotropic virus 1
(enzymology, genetics)
- Molecular Sequence Data
- Myxococcales
(genetics)
- Nucleic Acid Hybridization
- RNA, Bacterial
(analysis, biosynthesis, genetics)
- RNA-Directed DNA Polymerase
(genetics)
- Retroviridae
(enzymology, genetics)
- Ribonuclease H
- Sequence Homology, Nucleic Acid
- Transformation, Bacterial
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